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Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells
We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the isle...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167281/ https://www.ncbi.nlm.nih.gov/pubmed/30256861 http://dx.doi.org/10.4081/ejh.2009.e10 |
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author | Huang, Y.H. Sun, M.J. Jiang, M. Fu, B.Y. |
author_facet | Huang, Y.H. Sun, M.J. Jiang, M. Fu, B.Y. |
author_sort | Huang, Y.H. |
collection | PubMed |
description | We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells. |
format | Online Article Text |
id | pubmed-3167281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | PAGEPress Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-31672812011-11-09 Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells Huang, Y.H. Sun, M.J. Jiang, M. Fu, B.Y. Eur J Histochem Original Paper We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells. PAGEPress Publications 2009-06-29 /pmc/articles/PMC3167281/ /pubmed/30256861 http://dx.doi.org/10.4081/ejh.2009.e10 Text en ©2009 European Journal of Histochemistry |
spellingShingle | Original Paper Huang, Y.H. Sun, M.J. Jiang, M. Fu, B.Y. Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title_full | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title_fullStr | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title_full_unstemmed | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title_short | Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
title_sort | immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167281/ https://www.ncbi.nlm.nih.gov/pubmed/30256861 http://dx.doi.org/10.4081/ejh.2009.e10 |
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