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Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts

Bone is continuously repaired and remodelled through well-coordinated activity of osteoblasts that form new bone and osteoclasts, which resorb it. Osteoblasts synthesize and secrete two key molecules that are important for osteoclast differentiation, namely the ligand for the receptor of activator o...

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Autores principales: Narducci, P., Bortul, R., Bareggi, R., Nicolin, V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167292/
https://www.ncbi.nlm.nih.gov/pubmed/20353913
http://dx.doi.org/10.4081/ejh.2010.e6
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author Narducci, P.
Bortul, R.
Bareggi, R.
Nicolin, V.
author_facet Narducci, P.
Bortul, R.
Bareggi, R.
Nicolin, V.
author_sort Narducci, P.
collection PubMed
description Bone is continuously repaired and remodelled through well-coordinated activity of osteoblasts that form new bone and osteoclasts, which resorb it. Osteoblasts synthesize and secrete two key molecules that are important for osteoclast differentiation, namely the ligand for the receptor of activator of nuclear factor κB (RANKL) and its decoy receptor osteoprotegerin (OPG). Active membrane transport is a typical feature of the resorbing osteoclast during bone resorption. Normally, one resorption cycle takes several hours as observed by monitoring actin ring formation and consequent disappearance in vitro. During these cyclic changes, the cytoskeleton undergoes remarkable dynamic rearrangement. Active cells show a continuous process of exocytosis that plays an essential role in transport of membrane components, soluble molecules and receptor-mediated ligands thus allowing them to communicate with the environment. The processes that govern intracellular transport and trafficking in mature osteoclasts are poorly known. The principal methodological problem that have made these studies difficult is a physiological culture of osteoclasts that permit observing the vesicle apparatus in conditions similar to the in vivo conditions. In the present study we have used a number of morphological approaches to characterize the composition, formation and the endocytic and biosynthetic pathways that play roles in dynamics of differentiation of mature bone resorbing cells using a tri-dimensional system of physiologic coculture.
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spelling pubmed-31672922011-11-09 Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts Narducci, P. Bortul, R. Bareggi, R. Nicolin, V. Eur J Histochem Original Paper Bone is continuously repaired and remodelled through well-coordinated activity of osteoblasts that form new bone and osteoclasts, which resorb it. Osteoblasts synthesize and secrete two key molecules that are important for osteoclast differentiation, namely the ligand for the receptor of activator of nuclear factor κB (RANKL) and its decoy receptor osteoprotegerin (OPG). Active membrane transport is a typical feature of the resorbing osteoclast during bone resorption. Normally, one resorption cycle takes several hours as observed by monitoring actin ring formation and consequent disappearance in vitro. During these cyclic changes, the cytoskeleton undergoes remarkable dynamic rearrangement. Active cells show a continuous process of exocytosis that plays an essential role in transport of membrane components, soluble molecules and receptor-mediated ligands thus allowing them to communicate with the environment. The processes that govern intracellular transport and trafficking in mature osteoclasts are poorly known. The principal methodological problem that have made these studies difficult is a physiological culture of osteoclasts that permit observing the vesicle apparatus in conditions similar to the in vivo conditions. In the present study we have used a number of morphological approaches to characterize the composition, formation and the endocytic and biosynthetic pathways that play roles in dynamics of differentiation of mature bone resorbing cells using a tri-dimensional system of physiologic coculture. PAGEPress Publications 2010-03-20 /pmc/articles/PMC3167292/ /pubmed/20353913 http://dx.doi.org/10.4081/ejh.2010.e6 Text en ©Copyright P. Narducci et al., 2010 This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). Licensee PAGEPress, Italy
spellingShingle Original Paper
Narducci, P.
Bortul, R.
Bareggi, R.
Nicolin, V.
Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title_full Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title_fullStr Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title_full_unstemmed Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title_short Clathrin-dependent endocytosis of membrane-bound RANKL in differentiated osteoclasts
title_sort clathrin-dependent endocytosis of membrane-bound rankl in differentiated osteoclasts
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167292/
https://www.ncbi.nlm.nih.gov/pubmed/20353913
http://dx.doi.org/10.4081/ejh.2010.e6
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