Cargando…

Expression of SPANX proteins in normal prostatic tissue and in prostate cancer

The sperm protein associated with the nucleus in the X chromosome (SPANX) gene family encodes for proteins that are not only expressed in germ cells, but also in a number of tumors. In addition, SPANX genes map in an interval of the X chromosome (namely, Xq27), which has been found to be associated...

Descripción completa

Detalles Bibliográficos
Autores principales: Salemi, M., Calogero, A.E., Zaccarello, G., Castiglione, R., Cosentino, A., Catanuso, M., Vicari, E., Rappazzo, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167315/
https://www.ncbi.nlm.nih.gov/pubmed/20839415
http://dx.doi.org/10.4081/ejh.2010.e41
_version_ 1782211242421649408
author Salemi, M.
Calogero, A.E.
Zaccarello, G.
Castiglione, R.
Cosentino, A.
Catanuso, M.
Vicari, E.
Rappazzo, G.
author_facet Salemi, M.
Calogero, A.E.
Zaccarello, G.
Castiglione, R.
Cosentino, A.
Catanuso, M.
Vicari, E.
Rappazzo, G.
author_sort Salemi, M.
collection PubMed
description The sperm protein associated with the nucleus in the X chromosome (SPANX) gene family encodes for proteins that are not only expressed in germ cells, but also in a number of tumors. In addition, SPANX genes map in an interval of the X chromosome (namely, Xq27), which has been found to be associated with familial prostate cancer by linkage analysis. The aim of this study was therefore to evaluate SPANX protein expression in normal prostate tissues and in prostate carcinoma. For this purpose, formalin-fixed and paraffin-embedded sections obtained from 15 normal (at autopsy) donors and 12 men with prostate cancer were analyzed by immunohistochemistry. About 40% of both normal and tumor prostate samples resulted SPANX positive. Signals were exclusively within the nucleus in normal prostate cells, whereas both nuclear and cytoplasmic positivity was observed in tumor cells. In conclusion, these findings showed that SPANX genes are expressed in both normal and tumor prostate gland, but the latter showed a peculiar cytoplasmic staining positivity. This suggests a possible association between SPANX over expression and prostate cancer development. Additional studies are needed to corroborate this hypothesis.
format Online
Article
Text
id pubmed-3167315
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher PAGEPress Publications
record_format MEDLINE/PubMed
spelling pubmed-31673152011-11-09 Expression of SPANX proteins in normal prostatic tissue and in prostate cancer Salemi, M. Calogero, A.E. Zaccarello, G. Castiglione, R. Cosentino, A. Catanuso, M. Vicari, E. Rappazzo, G. Eur J Histochem Brief Note The sperm protein associated with the nucleus in the X chromosome (SPANX) gene family encodes for proteins that are not only expressed in germ cells, but also in a number of tumors. In addition, SPANX genes map in an interval of the X chromosome (namely, Xq27), which has been found to be associated with familial prostate cancer by linkage analysis. The aim of this study was therefore to evaluate SPANX protein expression in normal prostate tissues and in prostate carcinoma. For this purpose, formalin-fixed and paraffin-embedded sections obtained from 15 normal (at autopsy) donors and 12 men with prostate cancer were analyzed by immunohistochemistry. About 40% of both normal and tumor prostate samples resulted SPANX positive. Signals were exclusively within the nucleus in normal prostate cells, whereas both nuclear and cytoplasmic positivity was observed in tumor cells. In conclusion, these findings showed that SPANX genes are expressed in both normal and tumor prostate gland, but the latter showed a peculiar cytoplasmic staining positivity. This suggests a possible association between SPANX over expression and prostate cancer development. Additional studies are needed to corroborate this hypothesis. PAGEPress Publications 2010-09-26 /pmc/articles/PMC3167315/ /pubmed/20839415 http://dx.doi.org/10.4081/ejh.2010.e41 Text en ©Copyright M. Salemi et al., 2010 This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). Licensee PAGEPress, Italy
spellingShingle Brief Note
Salemi, M.
Calogero, A.E.
Zaccarello, G.
Castiglione, R.
Cosentino, A.
Catanuso, M.
Vicari, E.
Rappazzo, G.
Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title_full Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title_fullStr Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title_full_unstemmed Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title_short Expression of SPANX proteins in normal prostatic tissue and in prostate cancer
title_sort expression of spanx proteins in normal prostatic tissue and in prostate cancer
topic Brief Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167315/
https://www.ncbi.nlm.nih.gov/pubmed/20839415
http://dx.doi.org/10.4081/ejh.2010.e41
work_keys_str_mv AT salemim expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT calogeroae expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT zaccarellog expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT castiglioner expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT cosentinoa expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT catanusom expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT vicarie expressionofspanxproteinsinnormalprostatictissueandinprostatecancer
AT rappazzog expressionofspanxproteinsinnormalprostatictissueandinprostatecancer