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Koilocytes are enriched for alkaline-labile sites

This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA p...

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Autores principales: Cortés-Gutiérrez, E.I., Dávila-Rodríguez, M.I., Fernández, J.L., López-Fernández, C., Gosálvez, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167319/
https://www.ncbi.nlm.nih.gov/pubmed/21337807
http://dx.doi.org/10.4081/ejh.2010.e32
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author Cortés-Gutiérrez, E.I.
Dávila-Rodríguez, M.I.
Fernández, J.L.
López-Fernández, C.
Gosálvez, J.
author_facet Cortés-Gutiérrez, E.I.
Dávila-Rodríguez, M.I.
Fernández, J.L.
López-Fernández, C.
Gosálvez, J.
author_sort Cortés-Gutiérrez, E.I.
collection PubMed
description This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection.
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spelling pubmed-31673192011-11-09 Koilocytes are enriched for alkaline-labile sites Cortés-Gutiérrez, E.I. Dávila-Rodríguez, M.I. Fernández, J.L. López-Fernández, C. Gosálvez, J. Eur J Histochem Original Paper This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection. PAGEPress Publications 2010-12-21 /pmc/articles/PMC3167319/ /pubmed/21337807 http://dx.doi.org/10.4081/ejh.2010.e32 Text en ©Copyright E.I. Cortés-Gutiérrez et al., 2010 This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). Licensee PAGEPress, Italy
spellingShingle Original Paper
Cortés-Gutiérrez, E.I.
Dávila-Rodríguez, M.I.
Fernández, J.L.
López-Fernández, C.
Gosálvez, J.
Koilocytes are enriched for alkaline-labile sites
title Koilocytes are enriched for alkaline-labile sites
title_full Koilocytes are enriched for alkaline-labile sites
title_fullStr Koilocytes are enriched for alkaline-labile sites
title_full_unstemmed Koilocytes are enriched for alkaline-labile sites
title_short Koilocytes are enriched for alkaline-labile sites
title_sort koilocytes are enriched for alkaline-labile sites
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167319/
https://www.ncbi.nlm.nih.gov/pubmed/21337807
http://dx.doi.org/10.4081/ejh.2010.e32
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