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Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures
The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PAGEPress Publications
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167326/ https://www.ncbi.nlm.nih.gov/pubmed/21263745 http://dx.doi.org/10.4081/ejh.2010.e46 |
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author | Riccio, M. Resca, E. Maraldi, T. Pisciotta, A. Ferrari, A. Bruzzesi, G. De Pol, A. |
author_facet | Riccio, M. Resca, E. Maraldi, T. Pisciotta, A. Ferrari, A. Bruzzesi, G. De Pol, A. |
author_sort | Riccio, M. |
collection | PubMed |
description | The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D surface by using an osteogenic medium. During differentiation process, DPSCs express specific bone proteins like Runx-2, Osx, OPN and OCN with a sequential expression, analogous to those occurring during osteoblast differentiation, and produce extracellular calcium deposits. In order to differentiate cells in a 3D space that mimes the physiological environment, DPSCs were cultured in two distinct bioscaffolds, Matrigel™ and Collagen sponge. With the addition of a third dimension, osteogenic differentiation and mineralized extracellular matrix production significantly improved. In particular, in Matrigel™ DPSCs differentiated with osteoblast/osteocyte characteristics and connected by gap junction, and therefore formed calcified nodules with a 3D intercellular network. Furthermore, DPSCs differentiated in collagen sponge actively secrete human type I collagen micro-fibrils and form calcified matrix containing trabecular-like structures. These neo-formed DPSCs-scaffold devices may be used in regenerative surgical applications in order to resolve pathologies and traumas characterized by critical size bone defects. |
format | Online Article Text |
id | pubmed-3167326 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | PAGEPress Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-31673262011-11-09 Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures Riccio, M. Resca, E. Maraldi, T. Pisciotta, A. Ferrari, A. Bruzzesi, G. De Pol, A. Eur J Histochem Original Paper The aim of this study was to characterize the in vitro osteogenic differentiation of dental pulp stem cells (DPSCs) in 2D cultures and 3D biomaterials. DPSCs, separated from dental pulp by enzymatic digestion, and isolated by magnetic cell sorting were differentiated toward osteogenic lineage on 2D surface by using an osteogenic medium. During differentiation process, DPSCs express specific bone proteins like Runx-2, Osx, OPN and OCN with a sequential expression, analogous to those occurring during osteoblast differentiation, and produce extracellular calcium deposits. In order to differentiate cells in a 3D space that mimes the physiological environment, DPSCs were cultured in two distinct bioscaffolds, Matrigel™ and Collagen sponge. With the addition of a third dimension, osteogenic differentiation and mineralized extracellular matrix production significantly improved. In particular, in Matrigel™ DPSCs differentiated with osteoblast/osteocyte characteristics and connected by gap junction, and therefore formed calcified nodules with a 3D intercellular network. Furthermore, DPSCs differentiated in collagen sponge actively secrete human type I collagen micro-fibrils and form calcified matrix containing trabecular-like structures. These neo-formed DPSCs-scaffold devices may be used in regenerative surgical applications in order to resolve pathologies and traumas characterized by critical size bone defects. PAGEPress Publications 2010-12-21 /pmc/articles/PMC3167326/ /pubmed/21263745 http://dx.doi.org/10.4081/ejh.2010.e46 Text en ©Copyright M. Riccio et al., 2010 This work is licensed under a Creative Commons Attribution 3.0 License (by-nc 3.0). Licensee PAGEPress, Italy |
spellingShingle | Original Paper Riccio, M. Resca, E. Maraldi, T. Pisciotta, A. Ferrari, A. Bruzzesi, G. De Pol, A. Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title | Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title_full | Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title_fullStr | Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title_full_unstemmed | Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title_short | Human dental pulp stem cells produce mineralized matrix in 2D and 3D cultures |
title_sort | human dental pulp stem cells produce mineralized matrix in 2d and 3d cultures |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167326/ https://www.ncbi.nlm.nih.gov/pubmed/21263745 http://dx.doi.org/10.4081/ejh.2010.e46 |
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