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Cytokeratin 20-positive hepatocellular carcinoma

The differential diagnosis between hepatocellular carcinoma (HCC), cholangiocarcinoma (CC) and metastatic colorectal adenocarcinoma (MCA) may be difficult when only based on morphology. For this purpose immunohistochemical analyses are often required, utilizing antibodies directed against CK8-18, He...

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Autores principales: Fanni, D., Nemolato, S., Ganga, R., Senes, G., Gerosa, C., Van Eyken, P., Geboes, K., Faa, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167341/
https://www.ncbi.nlm.nih.gov/pubmed/22073364
http://dx.doi.org/10.4081/ejh.2009.e32
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author Fanni, D.
Nemolato, S.
Ganga, R.
Senes, G.
Gerosa, C.
Van Eyken, P.
Geboes, K.
Faa, G.
author_facet Fanni, D.
Nemolato, S.
Ganga, R.
Senes, G.
Gerosa, C.
Van Eyken, P.
Geboes, K.
Faa, G.
author_sort Fanni, D.
collection PubMed
description The differential diagnosis between hepatocellular carcinoma (HCC), cholangiocarcinoma (CC) and metastatic colorectal adenocarcinoma (MCA) may be difficult when only based on morphology. For this purpose immunohistochemical analyses are often required, utilizing antibodies directed against CK8-18, Hep-Par1, glypican 3, CK7, CK19, CK20. Here we report a case of a 65-year-old man who presented with a clinical picture of decompensated cirrhosis. Ultrasonography revealed two nodular areas in the right liver lobe. Liver needle biopsy revealed micro-macronodular cirrhosis associated with HCC with trabecular and pseudoglandular patterns. Immunohistochemically, tumour cells were diffusely positive for CK8-18 and also diffusely immunostained by glypican 3 and Hep-Par1. Interestingly, a diffuse and strong staining for CK20 was detected in the vast majority of tumor cells, particularly in the areas showing a pseudo-glandular pattern. No immunostaining for CK7 and CK19 was found in the tumor cells. The tumor behaved aggressively, with a rapid diffusion to the whole liver. The patient died from the disease few months after presentation. These findings underline that the interpretation of the expression of CK20 alone in the differential diagnosis among HCC, CC and MCA should be done with caution because a diffuse immunoreactivity for CK20 alone may not rule out the diagnosis of HCC.
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spelling pubmed-31673412011-11-09 Cytokeratin 20-positive hepatocellular carcinoma Fanni, D. Nemolato, S. Ganga, R. Senes, G. Gerosa, C. Van Eyken, P. Geboes, K. Faa, G. Eur J Histochem Brief Note The differential diagnosis between hepatocellular carcinoma (HCC), cholangiocarcinoma (CC) and metastatic colorectal adenocarcinoma (MCA) may be difficult when only based on morphology. For this purpose immunohistochemical analyses are often required, utilizing antibodies directed against CK8-18, Hep-Par1, glypican 3, CK7, CK19, CK20. Here we report a case of a 65-year-old man who presented with a clinical picture of decompensated cirrhosis. Ultrasonography revealed two nodular areas in the right liver lobe. Liver needle biopsy revealed micro-macronodular cirrhosis associated with HCC with trabecular and pseudoglandular patterns. Immunohistochemically, tumour cells were diffusely positive for CK8-18 and also diffusely immunostained by glypican 3 and Hep-Par1. Interestingly, a diffuse and strong staining for CK20 was detected in the vast majority of tumor cells, particularly in the areas showing a pseudo-glandular pattern. No immunostaining for CK7 and CK19 was found in the tumor cells. The tumor behaved aggressively, with a rapid diffusion to the whole liver. The patient died from the disease few months after presentation. These findings underline that the interpretation of the expression of CK20 alone in the differential diagnosis among HCC, CC and MCA should be done with caution because a diffuse immunoreactivity for CK20 alone may not rule out the diagnosis of HCC. PAGEPress Publications 2009-12-29 /pmc/articles/PMC3167341/ /pubmed/22073364 http://dx.doi.org/10.4081/ejh.2009.e32 Text en ©2009 European Journal of Histochemistry
spellingShingle Brief Note
Fanni, D.
Nemolato, S.
Ganga, R.
Senes, G.
Gerosa, C.
Van Eyken, P.
Geboes, K.
Faa, G.
Cytokeratin 20-positive hepatocellular carcinoma
title Cytokeratin 20-positive hepatocellular carcinoma
title_full Cytokeratin 20-positive hepatocellular carcinoma
title_fullStr Cytokeratin 20-positive hepatocellular carcinoma
title_full_unstemmed Cytokeratin 20-positive hepatocellular carcinoma
title_short Cytokeratin 20-positive hepatocellular carcinoma
title_sort cytokeratin 20-positive hepatocellular carcinoma
topic Brief Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167341/
https://www.ncbi.nlm.nih.gov/pubmed/22073364
http://dx.doi.org/10.4081/ejh.2009.e32
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