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Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods
Signal amplification is a key component of molecular detection. Enzyme-free signal amplification is especially appealing for the development of low-cost, point-of-care diagnostics. It has been previously shown that enzyme-free DNA circuits with signal-amplification capacity can be designed using a m...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167626/ https://www.ncbi.nlm.nih.gov/pubmed/21693555 http://dx.doi.org/10.1093/nar/gkr504 |
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author | Li, Bingling Ellington, Andrew D. Chen, Xi |
author_facet | Li, Bingling Ellington, Andrew D. Chen, Xi |
author_sort | Li, Bingling |
collection | PubMed |
description | Signal amplification is a key component of molecular detection. Enzyme-free signal amplification is especially appealing for the development of low-cost, point-of-care diagnostics. It has been previously shown that enzyme-free DNA circuits with signal-amplification capacity can be designed using a mechanism called ‘catalyzed hairpin assembly’. However, it is unclear whether the efficiency and modularity of such circuits is suitable for multiple analytical applications. We have therefore designed and characterized a simplified DNA circuit based on catalyzed hairpin assembly, and applied it to multiple different analytical formats, including fluorescent, colorimetric, and electrochemical and signaling. By optimizing the design of previous hairpin-based catalytic assemblies we found that our circuit has almost zero background and a high catalytic efficiency, with a k(cat) value above 1 min(−1). The inherent modularity of the circuit allowed us to readily adapt our circuit to detect both RNA and small molecule analytes. Overall, these data demonstrate that catalyzed hairpin assembly is suitable for analyte detection and signal amplification in a ‘plug-and-play’ fashion. |
format | Online Article Text |
id | pubmed-3167626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31676262011-09-06 Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods Li, Bingling Ellington, Andrew D. Chen, Xi Nucleic Acids Res Methods Online Signal amplification is a key component of molecular detection. Enzyme-free signal amplification is especially appealing for the development of low-cost, point-of-care diagnostics. It has been previously shown that enzyme-free DNA circuits with signal-amplification capacity can be designed using a mechanism called ‘catalyzed hairpin assembly’. However, it is unclear whether the efficiency and modularity of such circuits is suitable for multiple analytical applications. We have therefore designed and characterized a simplified DNA circuit based on catalyzed hairpin assembly, and applied it to multiple different analytical formats, including fluorescent, colorimetric, and electrochemical and signaling. By optimizing the design of previous hairpin-based catalytic assemblies we found that our circuit has almost zero background and a high catalytic efficiency, with a k(cat) value above 1 min(−1). The inherent modularity of the circuit allowed us to readily adapt our circuit to detect both RNA and small molecule analytes. Overall, these data demonstrate that catalyzed hairpin assembly is suitable for analyte detection and signal amplification in a ‘plug-and-play’ fashion. Oxford University Press 2011-09 2011-06-21 /pmc/articles/PMC3167626/ /pubmed/21693555 http://dx.doi.org/10.1093/nar/gkr504 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methods Online Li, Bingling Ellington, Andrew D. Chen, Xi Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title | Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title_full | Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title_fullStr | Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title_full_unstemmed | Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title_short | Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods |
title_sort | rational, modular adaptation of enzyme-free dna circuits to multiple detection methods |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167626/ https://www.ncbi.nlm.nih.gov/pubmed/21693555 http://dx.doi.org/10.1093/nar/gkr504 |
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