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In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection

BACKGROUND: Leishmania parasites have been reported to interfere and even subvert their host immune responses to enhance their chances of survival and proliferation. Experimental Leishmania infection in mice has been widely used in the identification of specific parasite virulence factors involved i...

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Autores principales: Pereira, Bernardo AS, Silva, Franklin S, Rebello, Karina M, Marín-Villa, Marcel, Traub-Cseko, Yara M, Andrade, Thereza CB, Bertho, Álvaro L, Caffarena, Ernesto R, Alves, Carlos R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167762/
https://www.ncbi.nlm.nih.gov/pubmed/21824434
http://dx.doi.org/10.1186/1471-2172-12-44
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author Pereira, Bernardo AS
Silva, Franklin S
Rebello, Karina M
Marín-Villa, Marcel
Traub-Cseko, Yara M
Andrade, Thereza CB
Bertho, Álvaro L
Caffarena, Ernesto R
Alves, Carlos R
author_facet Pereira, Bernardo AS
Silva, Franklin S
Rebello, Karina M
Marín-Villa, Marcel
Traub-Cseko, Yara M
Andrade, Thereza CB
Bertho, Álvaro L
Caffarena, Ernesto R
Alves, Carlos R
author_sort Pereira, Bernardo AS
collection PubMed
description BACKGROUND: Leishmania parasites have been reported to interfere and even subvert their host immune responses to enhance their chances of survival and proliferation. Experimental Leishmania infection in mice has been widely used in the identification of specific parasite virulence factors involved in the interaction with the host immune system. Cysteine-proteinase B (CPB) is an important virulence factor in parasites from the Leishmania (Leishmania) mexicana complex: it inhibits lymphocytes Th1 and/or promotes Th2 responses either through proteolytic activity or through epitopes derived from its COOH-terminal extension. In the present study we analyzed the effects of Leishmania (Leishmania) amazonensis CPB COOH-terminal extension-derived peptides on cell cultures from murine strains with distinct levels of susceptibility to infection: BALB/c, highly susceptible, and CBA, mildly resistant. RESULTS: Predicted epitopes, obtained by in silico mapping, displayed the ability to induce cell proliferation and expression of cytokines related to Th1 and Th2 responses. Furthermore, we applied in silico simulations to investigate how the MHC/epitopes interactions could be related to the immunomodulatory effects on cytokines, finding evidence that specific interaction patterns can be related to in vitro activities. CONCLUSIONS: Based on our results, we consider that some peptides from the CPB COOH-terminal extension may influence host immune responses in the murine infection, thus helping Leishmania survival.
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spelling pubmed-31677622011-09-07 In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection Pereira, Bernardo AS Silva, Franklin S Rebello, Karina M Marín-Villa, Marcel Traub-Cseko, Yara M Andrade, Thereza CB Bertho, Álvaro L Caffarena, Ernesto R Alves, Carlos R BMC Immunol Research Article BACKGROUND: Leishmania parasites have been reported to interfere and even subvert their host immune responses to enhance their chances of survival and proliferation. Experimental Leishmania infection in mice has been widely used in the identification of specific parasite virulence factors involved in the interaction with the host immune system. Cysteine-proteinase B (CPB) is an important virulence factor in parasites from the Leishmania (Leishmania) mexicana complex: it inhibits lymphocytes Th1 and/or promotes Th2 responses either through proteolytic activity or through epitopes derived from its COOH-terminal extension. In the present study we analyzed the effects of Leishmania (Leishmania) amazonensis CPB COOH-terminal extension-derived peptides on cell cultures from murine strains with distinct levels of susceptibility to infection: BALB/c, highly susceptible, and CBA, mildly resistant. RESULTS: Predicted epitopes, obtained by in silico mapping, displayed the ability to induce cell proliferation and expression of cytokines related to Th1 and Th2 responses. Furthermore, we applied in silico simulations to investigate how the MHC/epitopes interactions could be related to the immunomodulatory effects on cytokines, finding evidence that specific interaction patterns can be related to in vitro activities. CONCLUSIONS: Based on our results, we consider that some peptides from the CPB COOH-terminal extension may influence host immune responses in the murine infection, thus helping Leishmania survival. BioMed Central 2011-08-08 /pmc/articles/PMC3167762/ /pubmed/21824434 http://dx.doi.org/10.1186/1471-2172-12-44 Text en Copyright ©2011 Pereira et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pereira, Bernardo AS
Silva, Franklin S
Rebello, Karina M
Marín-Villa, Marcel
Traub-Cseko, Yara M
Andrade, Thereza CB
Bertho, Álvaro L
Caffarena, Ernesto R
Alves, Carlos R
In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title_full In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title_fullStr In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title_full_unstemmed In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title_short In silico predicted epitopes from the COOH-terminal extension of cysteine proteinase B inducing distinct immune responses during Leishmania (Leishmania) amazonensis experimental murine infection
title_sort in silico predicted epitopes from the cooh-terminal extension of cysteine proteinase b inducing distinct immune responses during leishmania (leishmania) amazonensis experimental murine infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167762/
https://www.ncbi.nlm.nih.gov/pubmed/21824434
http://dx.doi.org/10.1186/1471-2172-12-44
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