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Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis

BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis. AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and...

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Autores principales: Dominic, RM Saldanha, Prashanth, HV, Shenoy, Shalini, Baliga, Shrikala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167971/
https://www.ncbi.nlm.nih.gov/pubmed/21938253
http://dx.doi.org/10.4103/0974-2727.59702
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author Dominic, RM Saldanha
Prashanth, HV
Shenoy, Shalini
Baliga, Shrikala
author_facet Dominic, RM Saldanha
Prashanth, HV
Shenoy, Shalini
Baliga, Shrikala
author_sort Dominic, RM Saldanha
collection PubMed
description BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis. AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test. MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans. RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture. CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.
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spelling pubmed-31679712011-09-21 Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis Dominic, RM Saldanha Prashanth, HV Shenoy, Shalini Baliga, Shrikala J Lab Physicians Original Article BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis. AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test. MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans. RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture. CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients. Medknow Publications 2009 /pmc/articles/PMC3167971/ /pubmed/21938253 http://dx.doi.org/10.4103/0974-2727.59702 Text en © Journal of Laboratory Physicians http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Dominic, RM Saldanha
Prashanth, HV
Shenoy, Shalini
Baliga, Shrikala
Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title_full Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title_fullStr Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title_full_unstemmed Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title_short Diagnostic Value of Latex Agglutination in Cryptococcal Meningitis
title_sort diagnostic value of latex agglutination in cryptococcal meningitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3167971/
https://www.ncbi.nlm.nih.gov/pubmed/21938253
http://dx.doi.org/10.4103/0974-2727.59702
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