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Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene

Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence chara...

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Detalles Bibliográficos
Autores principales: Wang, Honggang, Sun, Wuzhuang, Li, Zhu, Wang, Xiufang, Lv, Zhanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168177/
https://www.ncbi.nlm.nih.gov/pubmed/21931509
http://dx.doi.org/10.1590/S1415-47572011005000018