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Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE

BACKGROUND: Mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. However, it has been difficult to define the precise effects of mineral...

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Autores principales: Altenbach, Susan B, Tanaka, Charlene K, Hurkman, William J, Whitehand, Linda C, Vensel, William H, Dupont, Frances M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168407/
https://www.ncbi.nlm.nih.gov/pubmed/21816081
http://dx.doi.org/10.1186/1477-5956-9-46
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author Altenbach, Susan B
Tanaka, Charlene K
Hurkman, William J
Whitehand, Linda C
Vensel, William H
Dupont, Frances M
author_facet Altenbach, Susan B
Tanaka, Charlene K
Hurkman, William J
Whitehand, Linda C
Vensel, William H
Dupont, Frances M
author_sort Altenbach, Susan B
collection PubMed
description BACKGROUND: Mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. However, it has been difficult to define the precise effects of mineral nutrition on protein composition because of the complexity of the wheat flour proteome. Recent improvements in the identification of flour proteins by tandem mass spectrometry (MS/MS) and the availability of a comprehensive proteome map of flour from the US wheat Butte 86 now make it possible to document changes in the proportions of individual flour proteins that result from the application of mineral nutrition. RESULTS: Plants of Triticum aestivum 'Butte 86' were grown with or without post-anthesis fertilization (PAF) and quantitative 2-dimensional gel electrophoresis (2-DE) was used to analyze protein composition of the resulting flour. Significant changes in the proportions of 54 unique proteins were observed as a result of the treatment. Most omega-gliadins, high molecular weight glutenin subunits (HMW-GS) and serpins as well as some alpha-gliadins increased in proportion with PAF. In contrast, alpha-amylase/protease inhibitors, farinins, purinins and puroindolines decreased in proportion. Decreases were also observed in several low molecular weight glutenin subunits (LMW-GS), globulins, defense proteins and enzymes. The ratio of HMW-GS to LMW-GS in the flour increased from 0.61 to 0.95 and the ratio of gliadins to glutenins increased from 1.02 to 1.30 with PAF. Because flour protein content doubled with PAF from 7 to 14%, most protein types actually increased in absolute amount (μg/mg flour protein). Data further suggest that flour proteins change with PAF according to their content of sulfur-containing amino acids Cys + Met. CONCLUSIONS: A 2-DE approach revealed changes in the wheat flour proteome due to PAF that are important for flour quality and immunogenic potential. The work forms a baseline for further studies of the effects of environmental variables on flour protein composition and provides clues about the regulation of specific flour protein genes. The study also is important for identifying targets for breeding programs and biotechnology efforts aimed at improving flour quality.
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spelling pubmed-31684072011-09-08 Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE Altenbach, Susan B Tanaka, Charlene K Hurkman, William J Whitehand, Linda C Vensel, William H Dupont, Frances M Proteome Sci Research BACKGROUND: Mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. However, it has been difficult to define the precise effects of mineral nutrition on protein composition because of the complexity of the wheat flour proteome. Recent improvements in the identification of flour proteins by tandem mass spectrometry (MS/MS) and the availability of a comprehensive proteome map of flour from the US wheat Butte 86 now make it possible to document changes in the proportions of individual flour proteins that result from the application of mineral nutrition. RESULTS: Plants of Triticum aestivum 'Butte 86' were grown with or without post-anthesis fertilization (PAF) and quantitative 2-dimensional gel electrophoresis (2-DE) was used to analyze protein composition of the resulting flour. Significant changes in the proportions of 54 unique proteins were observed as a result of the treatment. Most omega-gliadins, high molecular weight glutenin subunits (HMW-GS) and serpins as well as some alpha-gliadins increased in proportion with PAF. In contrast, alpha-amylase/protease inhibitors, farinins, purinins and puroindolines decreased in proportion. Decreases were also observed in several low molecular weight glutenin subunits (LMW-GS), globulins, defense proteins and enzymes. The ratio of HMW-GS to LMW-GS in the flour increased from 0.61 to 0.95 and the ratio of gliadins to glutenins increased from 1.02 to 1.30 with PAF. Because flour protein content doubled with PAF from 7 to 14%, most protein types actually increased in absolute amount (μg/mg flour protein). Data further suggest that flour proteins change with PAF according to their content of sulfur-containing amino acids Cys + Met. CONCLUSIONS: A 2-DE approach revealed changes in the wheat flour proteome due to PAF that are important for flour quality and immunogenic potential. The work forms a baseline for further studies of the effects of environmental variables on flour protein composition and provides clues about the regulation of specific flour protein genes. The study also is important for identifying targets for breeding programs and biotechnology efforts aimed at improving flour quality. BioMed Central 2011-08-04 /pmc/articles/PMC3168407/ /pubmed/21816081 http://dx.doi.org/10.1186/1477-5956-9-46 Text en Copyright ©2011 Altenbach et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Altenbach, Susan B
Tanaka, Charlene K
Hurkman, William J
Whitehand, Linda C
Vensel, William H
Dupont, Frances M
Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title_full Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title_fullStr Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title_full_unstemmed Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title_short Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
title_sort differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-de
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168407/
https://www.ncbi.nlm.nih.gov/pubmed/21816081
http://dx.doi.org/10.1186/1477-5956-9-46
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