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Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy
We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to constru...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168417/ https://www.ncbi.nlm.nih.gov/pubmed/21851585 http://dx.doi.org/10.1186/1746-4811-7-26 |
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author | Smith-Moritz, Andreia M Chern, Mawsheng Lao, Jeemeng Sze-To, Wing Hoi Heazlewood, Joshua L Ronald, Pamela C Vega-Sánchez, Miguel E |
author_facet | Smith-Moritz, Andreia M Chern, Mawsheng Lao, Jeemeng Sze-To, Wing Hoi Heazlewood, Joshua L Ronald, Pamela C Vega-Sánchez, Miguel E |
author_sort | Smith-Moritz, Andreia M |
collection | PubMed |
description | We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population. |
format | Online Article Text |
id | pubmed-3168417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31684172011-09-08 Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy Smith-Moritz, Andreia M Chern, Mawsheng Lao, Jeemeng Sze-To, Wing Hoi Heazlewood, Joshua L Ronald, Pamela C Vega-Sánchez, Miguel E Plant Methods Methodology We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population. BioMed Central 2011-08-18 /pmc/articles/PMC3168417/ /pubmed/21851585 http://dx.doi.org/10.1186/1746-4811-7-26 Text en Copyright ©2011 Smith-Moritz et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Smith-Moritz, Andreia M Chern, Mawsheng Lao, Jeemeng Sze-To, Wing Hoi Heazlewood, Joshua L Ronald, Pamela C Vega-Sánchez, Miguel E Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title | Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title_full | Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title_fullStr | Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title_full_unstemmed | Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title_short | Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy |
title_sort | combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by fourier transform near infrared spectroscopy |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168417/ https://www.ncbi.nlm.nih.gov/pubmed/21851585 http://dx.doi.org/10.1186/1746-4811-7-26 |
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