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Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System

The limited expression of N-Glycolyl GM3 (NeuGcGM3) ganglioside in human normal tissues, as well as its presence in melanoma and breast carcinoma using 14F7 Mab (anti-NeuGcGM3), has been previously reported. In this work we evaluated for the first time the 14F7 Mab immunorecognition in some digestiv...

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Autores principales: Blanco, Rancés, Rengifo, Enrique, Cedeño, Mercedes, Rengifo, Charles E., Alonso, Daniel F., Carr, Adriana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168460/
https://www.ncbi.nlm.nih.gov/pubmed/21991524
http://dx.doi.org/10.5402/2011/645641
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author Blanco, Rancés
Rengifo, Enrique
Cedeño, Mercedes
Rengifo, Charles E.
Alonso, Daniel F.
Carr, Adriana
author_facet Blanco, Rancés
Rengifo, Enrique
Cedeño, Mercedes
Rengifo, Charles E.
Alonso, Daniel F.
Carr, Adriana
author_sort Blanco, Rancés
collection PubMed
description The limited expression of N-Glycolyl GM3 (NeuGcGM3) ganglioside in human normal tissues, as well as its presence in melanoma and breast carcinoma using 14F7 Mab (anti-NeuGcGM3), has been previously reported. In this work we evaluated for the first time the 14F7 Mab immunorecognition in some digestive system tumors. Immunohistochemical assays were made with 14F7, followed by anti-mouse biotinylated antibody and ABC/HRP system in normal and pathological human tissues were made. No immunoreaction was evidenced in normal tissues. The reactivity of 14F7 was detected in all adenocarcinomas of the stomach (12/12), colon (12/12), and pancreas (11/11). A finely granular immunorecognition in esophageal tumors (5/15), epidermoid carcinoma of the rectum (5/7), and basaloid carcinoma (4/5) of the latter as well as in hepatocellular carcinoma (13/14) was also observed. Our results are in agreement with the assumption that NeuGcGM3 ganglioside may be considered as target for passive and active immunotherapy in digestive system malignancies expressing this molecule.
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spelling pubmed-31684602011-10-11 Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System Blanco, Rancés Rengifo, Enrique Cedeño, Mercedes Rengifo, Charles E. Alonso, Daniel F. Carr, Adriana ISRN Gastroenterol Research Article The limited expression of N-Glycolyl GM3 (NeuGcGM3) ganglioside in human normal tissues, as well as its presence in melanoma and breast carcinoma using 14F7 Mab (anti-NeuGcGM3), has been previously reported. In this work we evaluated for the first time the 14F7 Mab immunorecognition in some digestive system tumors. Immunohistochemical assays were made with 14F7, followed by anti-mouse biotinylated antibody and ABC/HRP system in normal and pathological human tissues were made. No immunoreaction was evidenced in normal tissues. The reactivity of 14F7 was detected in all adenocarcinomas of the stomach (12/12), colon (12/12), and pancreas (11/11). A finely granular immunorecognition in esophageal tumors (5/15), epidermoid carcinoma of the rectum (5/7), and basaloid carcinoma (4/5) of the latter as well as in hepatocellular carcinoma (13/14) was also observed. Our results are in agreement with the assumption that NeuGcGM3 ganglioside may be considered as target for passive and active immunotherapy in digestive system malignancies expressing this molecule. International Scholarly Research Network 2011 2010-12-02 /pmc/articles/PMC3168460/ /pubmed/21991524 http://dx.doi.org/10.5402/2011/645641 Text en Copyright © 2011 Rancés Blanco et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Blanco, Rancés
Rengifo, Enrique
Cedeño, Mercedes
Rengifo, Charles E.
Alonso, Daniel F.
Carr, Adriana
Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title_full Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title_fullStr Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title_full_unstemmed Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title_short Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Epithelial Malignant Tumors from Digestive System
title_sort immunoreactivity of the 14f7 mab raised against n-glycolyl gm3 ganglioside in epithelial malignant tumors from digestive system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168460/
https://www.ncbi.nlm.nih.gov/pubmed/21991524
http://dx.doi.org/10.5402/2011/645641
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