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Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library

Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs) might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones,...

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Autores principales: Yu, Seong-Lan, Jung, Woo-Young, Jung, Kie-Chul, Cho, In-Cheol, Lim, Hyun-Tae, Jin, Dong-Il, Lee, Jun-Heon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168785/
https://www.ncbi.nlm.nih.gov/pubmed/21912484
http://dx.doi.org/10.1155/2012/482568
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author Yu, Seong-Lan
Jung, Woo-Young
Jung, Kie-Chul
Cho, In-Cheol
Lim, Hyun-Tae
Jin, Dong-Il
Lee, Jun-Heon
author_facet Yu, Seong-Lan
Jung, Woo-Young
Jung, Kie-Chul
Cho, In-Cheol
Lim, Hyun-Tae
Jin, Dong-Il
Lee, Jun-Heon
author_sort Yu, Seong-Lan
collection PubMed
description Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs) might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR) region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones. Premature stop codons were observed in only three PERV-A clones. Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel. The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study. The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation.
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spelling pubmed-31687852011-09-12 Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library Yu, Seong-Lan Jung, Woo-Young Jung, Kie-Chul Cho, In-Cheol Lim, Hyun-Tae Jin, Dong-Il Lee, Jun-Heon J Biomed Biotechnol Research Article Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues. However, porcine endogenous retroviruses (PERVs) might transmit new infectious disease to humans during xenotransplantation. To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library. The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR) region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones. Premature stop codons were observed in only three PERV-A clones. Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel. The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study. The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation. Hindawi Publishing Corporation 2012 2011-09-07 /pmc/articles/PMC3168785/ /pubmed/21912484 http://dx.doi.org/10.1155/2012/482568 Text en Copyright © 2012 Seong-Lan Yu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Seong-Lan
Jung, Woo-Young
Jung, Kie-Chul
Cho, In-Cheol
Lim, Hyun-Tae
Jin, Dong-Il
Lee, Jun-Heon
Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title_full Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title_fullStr Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title_full_unstemmed Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title_short Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
title_sort characterization of porcine endogenous retrovirus clones from the nih miniature pig bac library
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168785/
https://www.ncbi.nlm.nih.gov/pubmed/21912484
http://dx.doi.org/10.1155/2012/482568
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