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Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients

Introduction: cytokines and their receptor genes are very polymorphic. SNPs in the promotor region of the gene may influence the rate of cytokine secretion and may affect the biological activity of the encoded cytokine. A number of cytokines and cytokine receptors have been directly linked to the de...

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Autores principales: Banu, C, Moise, A, Arion, CV, Coriu, D, T̆nase, A, Constantinescu, I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Carol Davila University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168821/
https://www.ncbi.nlm.nih.gov/pubmed/22567049
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author Banu, C
Moise, A
Arion, CV
Coriu, D
T̆nase, A
Constantinescu, I
author_facet Banu, C
Moise, A
Arion, CV
Coriu, D
T̆nase, A
Constantinescu, I
author_sort Banu, C
collection PubMed
description Introduction: cytokines and their receptor genes are very polymorphic. SNPs in the promotor region of the gene may influence the rate of cytokine secretion and may affect the biological activity of the encoded cytokine. A number of cytokines and cytokine receptors have been directly linked to the development of human cancers. The aim of our study was to determine the cytokine gene polymorphism in Romanian multiple myeloma patients. Material and methods: cytokine genotyping was performed in 80 patients and 100 healthy blood donors using molecular biology methods (SSP–Invitrogen, USA). Results: analyzing each polymorphic site, there was an increased frequency of the following genotypes in patients compared to control group: Interleukin–1beta (IL–1β) pos.+3962 TT, IL–12 pos.–1188 CC, gamma–Interferon (γ–IFN) pos.+874 AA, Transforming Growth Factor– beta1 (TGF– β1) codon10 TT, IL–2 pos.–330 TG and pos.+166 TT, Interleukin–4Receptor alpha (IL–4Rα) pos.–33 TC, IL–10 pos.–1082 GG and pos.–592 CC, IL𢀓6 pos.–174 GG. It should be noted that almost one third of multiple myeloma patients had IL–6 pos.–174 GG genotype and 62% IL–10 GCC haplotype. These identified haplotypes are high interleukins producer, and this fact was confirmed by serum IL–6 and IL–10 levels performed by ELISA and enhanced chemiluminiscence methods. Conclusion: these markers could be successfully used, together with other specific clinical and biological parameters, as reliable individualized prognostic factors in multiple myeloma patients.
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spelling pubmed-31688212012-05-07 Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients Banu, C Moise, A Arion, CV Coriu, D T̆nase, A Constantinescu, I J Med Life Original Article Introduction: cytokines and their receptor genes are very polymorphic. SNPs in the promotor region of the gene may influence the rate of cytokine secretion and may affect the biological activity of the encoded cytokine. A number of cytokines and cytokine receptors have been directly linked to the development of human cancers. The aim of our study was to determine the cytokine gene polymorphism in Romanian multiple myeloma patients. Material and methods: cytokine genotyping was performed in 80 patients and 100 healthy blood donors using molecular biology methods (SSP–Invitrogen, USA). Results: analyzing each polymorphic site, there was an increased frequency of the following genotypes in patients compared to control group: Interleukin–1beta (IL–1β) pos.+3962 TT, IL–12 pos.–1188 CC, gamma–Interferon (γ–IFN) pos.+874 AA, Transforming Growth Factor– beta1 (TGF– β1) codon10 TT, IL–2 pos.–330 TG and pos.+166 TT, Interleukin–4Receptor alpha (IL–4Rα) pos.–33 TC, IL–10 pos.–1082 GG and pos.–592 CC, IL𢀓6 pos.–174 GG. It should be noted that almost one third of multiple myeloma patients had IL–6 pos.–174 GG genotype and 62% IL–10 GCC haplotype. These identified haplotypes are high interleukins producer, and this fact was confirmed by serum IL–6 and IL–10 levels performed by ELISA and enhanced chemiluminiscence methods. Conclusion: these markers could be successfully used, together with other specific clinical and biological parameters, as reliable individualized prognostic factors in multiple myeloma patients. Carol Davila University Press 2011-08-15 2011-08-25 /pmc/articles/PMC3168821/ /pubmed/22567049 Text en ©Carol Davila University Press http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Banu, C
Moise, A
Arion, CV
Coriu, D
T̆nase, A
Constantinescu, I
Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title_full Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title_fullStr Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title_full_unstemmed Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title_short Cytokine Gene Polymorphisms support diagnostic monitoring of Romanian Multiple Myeloma patients
title_sort cytokine gene polymorphisms support diagnostic monitoring of romanian multiple myeloma patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3168821/
https://www.ncbi.nlm.nih.gov/pubmed/22567049
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