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A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions

This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide i...

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Detalles Bibliográficos
Autores principales: Sokolik, Charles W., Walker, Annie S., Nishioka, Gary M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169343/
https://www.ncbi.nlm.nih.gov/pubmed/21918601
http://dx.doi.org/10.4137/ACI.S7827
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author Sokolik, Charles W.
Walker, Annie S.
Nishioka, Gary M.
author_facet Sokolik, Charles W.
Walker, Annie S.
Nishioka, Gary M.
author_sort Sokolik, Charles W.
collection PubMed
description This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I(−) present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I(−) is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray.
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spelling pubmed-31693432011-09-14 A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions Sokolik, Charles W. Walker, Annie S. Nishioka, Gary M. Anal Chem Insights Rapid Communication This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I(−) present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I(−) is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray. Libertas Academica 2011-08-31 /pmc/articles/PMC3169343/ /pubmed/21918601 http://dx.doi.org/10.4137/ACI.S7827 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.
spellingShingle Rapid Communication
Sokolik, Charles W.
Walker, Annie S.
Nishioka, Gary M.
A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title_full A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title_fullStr A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title_full_unstemmed A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title_short A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
title_sort simple and sensitive assay for measuring very small volumes of microprinted solutions
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169343/
https://www.ncbi.nlm.nih.gov/pubmed/21918601
http://dx.doi.org/10.4137/ACI.S7827
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