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A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions
This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide i...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169343/ https://www.ncbi.nlm.nih.gov/pubmed/21918601 http://dx.doi.org/10.4137/ACI.S7827 |
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author | Sokolik, Charles W. Walker, Annie S. Nishioka, Gary M. |
author_facet | Sokolik, Charles W. Walker, Annie S. Nishioka, Gary M. |
author_sort | Sokolik, Charles W. |
collection | PubMed |
description | This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I(−) present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I(−) is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray. |
format | Online Article Text |
id | pubmed-3169343 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-31693432011-09-14 A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions Sokolik, Charles W. Walker, Annie S. Nishioka, Gary M. Anal Chem Insights Rapid Communication This study describes an extremely sensitive and simple assay to measure small volumes of solutions, <1 nL. The assay takes advantage of the Sandell-Kolthoff reaction in which yellow cerium(IV) is reduced to colorless cerium(III) in the presence of arsenic(III) and catalytic quantities of iodide ion. The reaction is linear with respect to the rate of Ce(IV) reduction and the quantity of I(−) present. Typical assays can measure 10–100 picomoles of iodide in a sample. When I(−) is substituted for chloride ion in standard biological buffers, such as Tris-buffered saline, the assay can be used to determine the volume of solution printed in a microarray. Libertas Academica 2011-08-31 /pmc/articles/PMC3169343/ /pubmed/21918601 http://dx.doi.org/10.4137/ACI.S7827 Text en © the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited. |
spellingShingle | Rapid Communication Sokolik, Charles W. Walker, Annie S. Nishioka, Gary M. A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title | A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title_full | A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title_fullStr | A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title_full_unstemmed | A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title_short | A Simple and Sensitive Assay for Measuring Very Small Volumes of Microprinted Solutions |
title_sort | simple and sensitive assay for measuring very small volumes of microprinted solutions |
topic | Rapid Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169343/ https://www.ncbi.nlm.nih.gov/pubmed/21918601 http://dx.doi.org/10.4137/ACI.S7827 |
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