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Gene Composer in a structural genomics environment

The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino-acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high-throughput protei...

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Autores principales: Lorimer, Don, Raymond, Amy, Mixon, Mark, Burgin, Alex, Staker, Bart, Stewart, Lance
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169390/
https://www.ncbi.nlm.nih.gov/pubmed/21904038
http://dx.doi.org/10.1107/S1744309111027424
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author Lorimer, Don
Raymond, Amy
Mixon, Mark
Burgin, Alex
Staker, Bart
Stewart, Lance
author_facet Lorimer, Don
Raymond, Amy
Mixon, Mark
Burgin, Alex
Staker, Bart
Stewart, Lance
author_sort Lorimer, Don
collection PubMed
description The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino-acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high-throughput protein structure determination, a database software package, Gene Composer, has been developed which facilitates the information-rich design of protein constructs and their underlying gene sequences. With its modular workflow design and numerous graphical user interfaces, Gene Composer enables researchers to perform all common bioinformatics steps used in modern structure-guided protein engineering and synthetic gene engineering. An example of the structure determination of H1N1 RNA-dependent RNA polymerase PB2 subunit is given.
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spelling pubmed-31693902011-09-21 Gene Composer in a structural genomics environment Lorimer, Don Raymond, Amy Mixon, Mark Burgin, Alex Staker, Bart Stewart, Lance Acta Crystallogr Sect F Struct Biol Cryst Commun Laboratory Communications The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino-acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high-throughput protein structure determination, a database software package, Gene Composer, has been developed which facilitates the information-rich design of protein constructs and their underlying gene sequences. With its modular workflow design and numerous graphical user interfaces, Gene Composer enables researchers to perform all common bioinformatics steps used in modern structure-guided protein engineering and synthetic gene engineering. An example of the structure determination of H1N1 RNA-dependent RNA polymerase PB2 subunit is given. International Union of Crystallography 2011-08-13 /pmc/articles/PMC3169390/ /pubmed/21904038 http://dx.doi.org/10.1107/S1744309111027424 Text en © Lorimer et al. 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Laboratory Communications
Lorimer, Don
Raymond, Amy
Mixon, Mark
Burgin, Alex
Staker, Bart
Stewart, Lance
Gene Composer in a structural genomics environment
title Gene Composer in a structural genomics environment
title_full Gene Composer in a structural genomics environment
title_fullStr Gene Composer in a structural genomics environment
title_full_unstemmed Gene Composer in a structural genomics environment
title_short Gene Composer in a structural genomics environment
title_sort gene composer in a structural genomics environment
topic Laboratory Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169390/
https://www.ncbi.nlm.nih.gov/pubmed/21904038
http://dx.doi.org/10.1107/S1744309111027424
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