Cargando…

Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a gr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hewitt, Stephen N., Choi, Ryan, Kelley, Angela, Crowther, Gregory J., Napuli, Alberto J., Van Voorhis, Wesley C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2011
Materias:	Laboratory Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169393/ https://www.ncbi.nlm.nih.gov/pubmed/21904041 http://dx.doi.org/10.1107/S1744309111022159

Ejemplares similares

High-throughput protein production and purification at the Seattle Structural Genomics Center for Infectious Disease
por: Bryan, Cassie M., et al.
Publicado: (2011)

Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
por: Choi, Ryan, et al.
Publicado: (2011)

The Protein Maker: an automated system for high-throughput parallel purification
por: Smith, Eric R., et al.
Publicado: (2011)

Maltose-Binding Protein Fusion Allows for High Level Bacterial Expression and Purification of Bioactive Mammalian Cytokine Derivatives
por: Pennati, Andrea, et al.
Publicado: (2014)

Wheat germ cell-free expression system as a pathway to improve protein yield and solubility for the SSGCID pipeline
por: Guild, Katherine, et al.
Publicado: (2011)

A Research‐inspired biochemistry laboratory module–combining expression, purification, crystallization, structure‐solving, and characterization of a flavodoxin‐like protein
por: Hammerstad, Marta, et al.
Publicado: (2019)

Heterologous Expression of Mycobacterial Esx Complexes in Escherichia coli for Structural Studies Is Facilitated by the Use of Maltose Binding Protein Fusions
por: Arbing, Mark A., et al.
Publicado: (2013)

Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion
por: Park, Sangsu, et al.
Publicado: (2021)

Enhanced Expression and Purification of Membrane Proteins by SUMO Fusion in Escherichia coli
por: Zuo, Xun, et al.
Publicado: (2005)

Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
por: Nguyen, Anh Ngoc, et al.
Publicado: (2017)

Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins
por: Bokhove, Marcel, et al.
Publicado: (2016)

Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag
por: Nguyen, Minh Tan, et al.
Publicado: (2016)

High-Throughput Protein Expression and Purification for Proteomics Research
por: Doyle, Sharon A., et al.
Publicado: (2002)

Prokaryotic Soluble Overexpression and Purification of Bioactive Human Growth Hormone by Fusion to Thioredoxin, Maltose Binding Protein, and Protein Disulfide Isomerase
por: Nguyen, Minh Tan, et al.
Publicado: (2014)

Correction: Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag
por: Nguyen, Minh Tan, et al.
Publicado: (2017)

Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems
por: Reuten, Raphael, et al.
Publicado: (2016)

Expression, purification and characterization of soluble red rooster laforin as a fusion protein in Escherichia coli
por: Brewer, M Kathryn, et al.
Publicado: (2014)

Expression and purification of turkey coronavirus nucleocapsid protein in Escherichia coli
por: Loa, C.C., et al.
Publicado: (2004)

Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics
por: Christensen, Jeff, et al.
Publicado: (2011)

Gene design, cloning and protein-expression methods for high-value targets at the Seattle Structural Genomics Center for Infectious Disease
por: Raymond, Amy, et al.
Publicado: (2011)

Expression and purification of SARS coronavirus proteins using SUMO-fusions
por: Zuo, Xun, et al.
Publicado: (2005)

High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli
por: Wang, Huiyan, et al.
Publicado: (2010)

Semi-Quantitative Analyses of Hippocampal Heat Shock Protein-70 Expression Based on the Duration of Ischemia and the Volume of Cerebral Infarction in Mice
por: Choi, Jong-Il, et al.
Publicado: (2014)

Purity of maltose-binding protein – Recombinant streptavidin expressed in Escherichia coli BL21 (pD861-MBP: 327892)
por: Subroto, Toto, et al.
Publicado: (2022)

Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis
por: Buchko, Garry W., et al.
Publicado: (2011)

N-acetylcysteine suppresses colistimethate sodium-induced nephrotoxicity via activation of SOD2, eNOS, and MMP3 protein expressions
por: Ceylan, Bahadir, et al.
Publicado: (2018)

Homologous high-throughput expression and purification of highly conserved E coli proteins
por: Ergin, Asgar, et al.
Publicado: (2007)

The effect of cordycepin on brain oxidative stress and protein expression in streptozotocin-induced diabetic mice
por: SRISUKSAI, Krittika, et al.
Publicado: (2021)

Structure of an Engineered β-Lactamase Maltose Binding Protein Fusion Protein: Insights into Heterotropic Allosteric Regulation
por: Ke, Wei, et al.
Publicado: (2012)

Changes E3 ubiquitin protein ligase 1 gene mRNA expression correlated with IgA1 glycosylation in patients with IgA nephropathy
por: Liu, Youxia, et al.
Publicado: (2019)

Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
por: Malik, Ajamaluddin
Publicado: (2016)

Soluble Prokaryotic Overexpression and Purification of Bioactive Human Granulocyte Colony-Stimulating Factor by Maltose Binding Protein and Protein Disulfide Isomerase
por: Do, Bich Hang, et al.
Publicado: (2014)

Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli
por: Hollinger, Jared, et al.
Publicado: (2023)

Expression, purification, and cytotoxic evaluation of IL24-BR2 fusion protein
por: Pourhadi, Marjan, et al.
Publicado: (2019)

Serum insulin-like growth factor-binding protein 2 levels as an indicator for disease severity in enterohemorrhagic Escherichia coli induced hemolytic uremic syndrome
por: Tasaki, Yuko, et al.
Publicado: (2021)

Proteomic Identification of Proteins Differentially Expressed in Response to Resveratrol Treatment in Middle Cerebral Artery Occlusion Stroke Model
por: SHAH, Fawad-Ali, et al.
Publicado: (2014)

Exploration of twin‐arginine translocation for expression and purification of correctly folded proteins in Escherichia coli
por: Fisher, Adam C., et al.
Publicado: (2008)

High Level Expression and Purification of Recombinant Proteins from Escherichia coli with AK-TAG
por: Luo, Dan, et al.
Publicado: (2016)

Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli
por: Jha, Roshan, et al.
Publicado: (2017)

Expression, purification and characterization of the dimeric protruding domain of Macrobrachium rosenbergii nodavirus capsid protein expressed in Escherichia coli
por: Chong, Li Chuin, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_bq8jkqo7l4can4o8jphsce3ngm