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The Protein Maker: an automated system for high-throughput parallel purification

The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. T...

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Autores principales: Smith, Eric R., Begley, Darren W., Anderson, Vanessa, Raymond, Amy C., Haffner, Taryn E., Robinson, John I., Edwards, Thomas E., Duncan, Natalie, Gerdts, Cory J., Mixon, Mark B., Nollert, Peter, Staker, Bart L., Stewart, Lance J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169395/
https://www.ncbi.nlm.nih.gov/pubmed/21904043
http://dx.doi.org/10.1107/S1744309111028776
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author Smith, Eric R.
Begley, Darren W.
Anderson, Vanessa
Raymond, Amy C.
Haffner, Taryn E.
Robinson, John I.
Edwards, Thomas E.
Duncan, Natalie
Gerdts, Cory J.
Mixon, Mark B.
Nollert, Peter
Staker, Bart L.
Stewart, Lance J.
author_facet Smith, Eric R.
Begley, Darren W.
Anderson, Vanessa
Raymond, Amy C.
Haffner, Taryn E.
Robinson, John I.
Edwards, Thomas E.
Duncan, Natalie
Gerdts, Cory J.
Mixon, Mark B.
Nollert, Peter
Staker, Bart L.
Stewart, Lance J.
author_sort Smith, Eric R.
collection PubMed
description The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications.
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spelling pubmed-31693952011-09-21 The Protein Maker: an automated system for high-throughput parallel purification Smith, Eric R. Begley, Darren W. Anderson, Vanessa Raymond, Amy C. Haffner, Taryn E. Robinson, John I. Edwards, Thomas E. Duncan, Natalie Gerdts, Cory J. Mixon, Mark B. Nollert, Peter Staker, Bart L. Stewart, Lance J. Acta Crystallogr Sect F Struct Biol Cryst Commun Laboratory Communications The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications. International Union of Crystallography 2011-08-13 /pmc/articles/PMC3169395/ /pubmed/21904043 http://dx.doi.org/10.1107/S1744309111028776 Text en © Smith et al. 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Laboratory Communications
Smith, Eric R.
Begley, Darren W.
Anderson, Vanessa
Raymond, Amy C.
Haffner, Taryn E.
Robinson, John I.
Edwards, Thomas E.
Duncan, Natalie
Gerdts, Cory J.
Mixon, Mark B.
Nollert, Peter
Staker, Bart L.
Stewart, Lance J.
The Protein Maker: an automated system for high-throughput parallel purification
title The Protein Maker: an automated system for high-throughput parallel purification
title_full The Protein Maker: an automated system for high-throughput parallel purification
title_fullStr The Protein Maker: an automated system for high-throughput parallel purification
title_full_unstemmed The Protein Maker: an automated system for high-throughput parallel purification
title_short The Protein Maker: an automated system for high-throughput parallel purification
title_sort protein maker: an automated system for high-throughput parallel purification
topic Laboratory Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169395/
https://www.ncbi.nlm.nih.gov/pubmed/21904043
http://dx.doi.org/10.1107/S1744309111028776
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