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H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing
BACKGROUND: Endogenous retroviruses (ERVs) are parasitic sequences whose derepression is associated with cancer and genomic instability. Many ERV families are silenced in mouse embryonic stem cells (mESCs) via SETDB1-deposited trimethylated lysine 9 of histone 3 (H3K9me3), but the mechanism of H3K9m...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169442/ https://www.ncbi.nlm.nih.gov/pubmed/21774827 http://dx.doi.org/10.1186/1756-8935-4-12 |
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author | Maksakova, Irina A Goyal, Preeti Bullwinkel, Jörn Brown, Jeremy P Bilenky, Misha Mager, Dixie L Singh, Prim B Lorincz, Matthew C |
author_facet | Maksakova, Irina A Goyal, Preeti Bullwinkel, Jörn Brown, Jeremy P Bilenky, Misha Mager, Dixie L Singh, Prim B Lorincz, Matthew C |
author_sort | Maksakova, Irina A |
collection | PubMed |
description | BACKGROUND: Endogenous retroviruses (ERVs) are parasitic sequences whose derepression is associated with cancer and genomic instability. Many ERV families are silenced in mouse embryonic stem cells (mESCs) via SETDB1-deposited trimethylated lysine 9 of histone 3 (H3K9me3), but the mechanism of H3K9me3-dependent repression remains unknown. Multiple proteins, including members of the heterochromatin protein 1 (HP1) family, bind H3K9me2/3 and are involved in transcriptional silencing in model organisms. In this work, we address the role of such H3K9me2/3 "readers" in the silencing of ERVs in mESCs. RESULTS: We demonstrate that despite the reported function of HP1 proteins in H3K9me-dependent gene repression and the critical role of H3K9me3 in transcriptional silencing of class I and class II ERVs, the depletion of HP1α, HP1β and HP1γ, alone or in combination, is not sufficient for derepression of these elements in mESCs. While loss of HP1α or HP1β leads to modest defects in DNA methylation of ERVs or spreading of H4K20me3 into flanking genomic sequence, respectively, neither protein affects H3K9me3 or H4K20me3 in ERV bodies. Furthermore, using novel ERV reporter constructs targeted to a specific genomic site, we demonstrate that, relative to Setdb1, knockdown of the remaining known H3K9me3 readers expressed in mESCs, including Cdyl, Cdyl2, Cbx2, Cbx7, Mpp8, Uhrf1 and Jarid1a-c, leads to only modest proviral reactivation. CONCLUSION: Taken together, these results reveal that each of the known H3K9me3-binding proteins is dispensable for SETDB1-mediated ERV silencing. We speculate that H3K9me3 might maintain ERVs in a silent state in mESCs by directly inhibiting deposition of active covalent histone marks. |
format | Online Article Text |
id | pubmed-3169442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31694422011-09-09 H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing Maksakova, Irina A Goyal, Preeti Bullwinkel, Jörn Brown, Jeremy P Bilenky, Misha Mager, Dixie L Singh, Prim B Lorincz, Matthew C Epigenetics Chromatin Research BACKGROUND: Endogenous retroviruses (ERVs) are parasitic sequences whose derepression is associated with cancer and genomic instability. Many ERV families are silenced in mouse embryonic stem cells (mESCs) via SETDB1-deposited trimethylated lysine 9 of histone 3 (H3K9me3), but the mechanism of H3K9me3-dependent repression remains unknown. Multiple proteins, including members of the heterochromatin protein 1 (HP1) family, bind H3K9me2/3 and are involved in transcriptional silencing in model organisms. In this work, we address the role of such H3K9me2/3 "readers" in the silencing of ERVs in mESCs. RESULTS: We demonstrate that despite the reported function of HP1 proteins in H3K9me-dependent gene repression and the critical role of H3K9me3 in transcriptional silencing of class I and class II ERVs, the depletion of HP1α, HP1β and HP1γ, alone or in combination, is not sufficient for derepression of these elements in mESCs. While loss of HP1α or HP1β leads to modest defects in DNA methylation of ERVs or spreading of H4K20me3 into flanking genomic sequence, respectively, neither protein affects H3K9me3 or H4K20me3 in ERV bodies. Furthermore, using novel ERV reporter constructs targeted to a specific genomic site, we demonstrate that, relative to Setdb1, knockdown of the remaining known H3K9me3 readers expressed in mESCs, including Cdyl, Cdyl2, Cbx2, Cbx7, Mpp8, Uhrf1 and Jarid1a-c, leads to only modest proviral reactivation. CONCLUSION: Taken together, these results reveal that each of the known H3K9me3-binding proteins is dispensable for SETDB1-mediated ERV silencing. We speculate that H3K9me3 might maintain ERVs in a silent state in mESCs by directly inhibiting deposition of active covalent histone marks. BioMed Central 2011-07-20 /pmc/articles/PMC3169442/ /pubmed/21774827 http://dx.doi.org/10.1186/1756-8935-4-12 Text en Copyright ©2011 Maksakova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Maksakova, Irina A Goyal, Preeti Bullwinkel, Jörn Brown, Jeremy P Bilenky, Misha Mager, Dixie L Singh, Prim B Lorincz, Matthew C H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title | H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title_full | H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title_fullStr | H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title_full_unstemmed | H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title_short | H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing |
title_sort | h3k9me3-binding proteins are dispensable for setdb1/h3k9me3-dependent retroviral silencing |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169442/ https://www.ncbi.nlm.nih.gov/pubmed/21774827 http://dx.doi.org/10.1186/1756-8935-4-12 |
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