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Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice

BACKGROUND: Multi-walled carbon nanotubes (MWCNTs) are widely used in many disciplines due to their unique physical and chemical properties. Therefore, some concerns about the possible human health and environmental impacts of manufactured MWCNTs are rising. We hypothesized that instillation of MWCN...

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Autores principales: Wang, Xiaojia, Katwa, Pranita, Podila, Ramakrishna, Chen, Pengyu, Ke, Pu Chun, Rao, Apparao M, Walters, Dianne M, Wingard, Christopher J, Brown, Jared M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170188/
https://www.ncbi.nlm.nih.gov/pubmed/21851604
http://dx.doi.org/10.1186/1743-8977-8-24
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author Wang, Xiaojia
Katwa, Pranita
Podila, Ramakrishna
Chen, Pengyu
Ke, Pu Chun
Rao, Apparao M
Walters, Dianne M
Wingard, Christopher J
Brown, Jared M
author_facet Wang, Xiaojia
Katwa, Pranita
Podila, Ramakrishna
Chen, Pengyu
Ke, Pu Chun
Rao, Apparao M
Walters, Dianne M
Wingard, Christopher J
Brown, Jared M
author_sort Wang, Xiaojia
collection PubMed
description BACKGROUND: Multi-walled carbon nanotubes (MWCNTs) are widely used in many disciplines due to their unique physical and chemical properties. Therefore, some concerns about the possible human health and environmental impacts of manufactured MWCNTs are rising. We hypothesized that instillation of MWCNTs impairs pulmonary function in C57BL/6 mice due to development of lung inflammation and fibrosis. METHODS: MWCNTs were administered to C57BL/6 mice by oropharyngeal aspiration (1, 2, and 4 mg/kg) and we assessed lung inflammation and fibrosis by inflammatory cell infiltration, collagen content, and histological assessment. Pulmonary function was assessed using a FlexiVent system and levels of Ccl3, Ccl11, Mmp13 and IL-33 were measured by RT-PCR and ELISA. RESULTS: Mice administered MWCNTs exhibited increased inflammatory cell infiltration, collagen deposition and granuloma formation in lung tissue, which correlated with impaired pulmonary function as assessed by increased resistance, tissue damping, and decreased lung compliance. Pulmonary exposure to MWCNTs induced an inflammatory signature marked by cytokine (IL-33), chemokine (Ccl3 and Ccl11), and protease production (Mmp13) that promoted the inflammatory and fibrotic changes observed within the lung. CONCLUSIONS: These results further highlight the potential adverse health effects that may occur following MWCNT exposure and therefore we suggest these materials may pose a significant risk leading to impaired lung function following environmental and occupational exposures.
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spelling pubmed-31701882011-09-10 Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice Wang, Xiaojia Katwa, Pranita Podila, Ramakrishna Chen, Pengyu Ke, Pu Chun Rao, Apparao M Walters, Dianne M Wingard, Christopher J Brown, Jared M Part Fibre Toxicol Research BACKGROUND: Multi-walled carbon nanotubes (MWCNTs) are widely used in many disciplines due to their unique physical and chemical properties. Therefore, some concerns about the possible human health and environmental impacts of manufactured MWCNTs are rising. We hypothesized that instillation of MWCNTs impairs pulmonary function in C57BL/6 mice due to development of lung inflammation and fibrosis. METHODS: MWCNTs were administered to C57BL/6 mice by oropharyngeal aspiration (1, 2, and 4 mg/kg) and we assessed lung inflammation and fibrosis by inflammatory cell infiltration, collagen content, and histological assessment. Pulmonary function was assessed using a FlexiVent system and levels of Ccl3, Ccl11, Mmp13 and IL-33 were measured by RT-PCR and ELISA. RESULTS: Mice administered MWCNTs exhibited increased inflammatory cell infiltration, collagen deposition and granuloma formation in lung tissue, which correlated with impaired pulmonary function as assessed by increased resistance, tissue damping, and decreased lung compliance. Pulmonary exposure to MWCNTs induced an inflammatory signature marked by cytokine (IL-33), chemokine (Ccl3 and Ccl11), and protease production (Mmp13) that promoted the inflammatory and fibrotic changes observed within the lung. CONCLUSIONS: These results further highlight the potential adverse health effects that may occur following MWCNT exposure and therefore we suggest these materials may pose a significant risk leading to impaired lung function following environmental and occupational exposures. BioMed Central 2011-08-18 /pmc/articles/PMC3170188/ /pubmed/21851604 http://dx.doi.org/10.1186/1743-8977-8-24 Text en Copyright ©2011 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wang, Xiaojia
Katwa, Pranita
Podila, Ramakrishna
Chen, Pengyu
Ke, Pu Chun
Rao, Apparao M
Walters, Dianne M
Wingard, Christopher J
Brown, Jared M
Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title_full Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title_fullStr Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title_full_unstemmed Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title_short Multi-walled carbon nanotube instillation impairs pulmonary function in C57BL/6 mice
title_sort multi-walled carbon nanotube instillation impairs pulmonary function in c57bl/6 mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170188/
https://www.ncbi.nlm.nih.gov/pubmed/21851604
http://dx.doi.org/10.1186/1743-8977-8-24
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