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Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus

BACKGROUND: Neuraminidase (NA) inhibitors used for influenza therapy are believed to prevent the release of progeny virus from the surface of an infected cell. In this study, we found that NA inhibitors have a novel antiviral function against an avian influenza virus. RESULTS: Madin-Darby canine kid...

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Autores principales: Ushirogawa, Hiroshi, Ohuchi, Masanobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170304/
https://www.ncbi.nlm.nih.gov/pubmed/21851647
http://dx.doi.org/10.1186/1743-422X-8-411
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author Ushirogawa, Hiroshi
Ohuchi, Masanobu
author_facet Ushirogawa, Hiroshi
Ohuchi, Masanobu
author_sort Ushirogawa, Hiroshi
collection PubMed
description BACKGROUND: Neuraminidase (NA) inhibitors used for influenza therapy are believed to prevent the release of progeny virus from the surface of an infected cell. In this study, we found that NA inhibitors have a novel antiviral function against an avian influenza virus. RESULTS: Madin-Darby canine kidney cells, commonly used for the isolation and propagation of the influenza virus, were infected with an avian influenza viral strain A/chicken/German/N/49(H10N7) (H10/chicken) or a human influenza viral strain A/Osaka/981/98(H3N2) (H3/Osaka) virus. Cells were incubated in a medium without or with a NA inhibitor, oseltamivir carboxylate (GS4071), from 1 to 13 h post infection (p.i.). Infected cells were washed 12 h p.i. to remove GS4071, incubated for 1 h without GS4071, and assayed for virus production. Incubation with GS4071 decreased the production of infectious viruses. When H10/chicken virus-infected cells were incubated with GS4071 from 12 to 13 h p.i. (i.e., 1 h before the virus production assay), the inhibitory effect was clearly observed, however, the same was not evident for H3/Osaka virus-infected cells. Furthermore, viral protein synthesis in infected cells was not affected by GS4071. Using a scanning electron microscope, many single spherical buds were observed on the surface of H3/Osaka virus-infected cells incubated without GS4071, whereas many aggregated particles were observed on the surface of cells incubated with GS4071. However, many long tubular virus-like structures, with no aggregated particles, were observed on the surface of H10/chicken virus-infected cells incubated with GS4071. The same results were obtained when another NA inhibitor, zanamivir, was used. CONCLUSIONS: These results indicate that NA inhibitors interfered with virus particle formation in the H10/chicken virus-infected cells, in which the inhibitor caused the formation of long tubular virus-like structures instead of spherical virus particles.
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spelling pubmed-31703042011-09-10 Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus Ushirogawa, Hiroshi Ohuchi, Masanobu Virol J Research BACKGROUND: Neuraminidase (NA) inhibitors used for influenza therapy are believed to prevent the release of progeny virus from the surface of an infected cell. In this study, we found that NA inhibitors have a novel antiviral function against an avian influenza virus. RESULTS: Madin-Darby canine kidney cells, commonly used for the isolation and propagation of the influenza virus, were infected with an avian influenza viral strain A/chicken/German/N/49(H10N7) (H10/chicken) or a human influenza viral strain A/Osaka/981/98(H3N2) (H3/Osaka) virus. Cells were incubated in a medium without or with a NA inhibitor, oseltamivir carboxylate (GS4071), from 1 to 13 h post infection (p.i.). Infected cells were washed 12 h p.i. to remove GS4071, incubated for 1 h without GS4071, and assayed for virus production. Incubation with GS4071 decreased the production of infectious viruses. When H10/chicken virus-infected cells were incubated with GS4071 from 12 to 13 h p.i. (i.e., 1 h before the virus production assay), the inhibitory effect was clearly observed, however, the same was not evident for H3/Osaka virus-infected cells. Furthermore, viral protein synthesis in infected cells was not affected by GS4071. Using a scanning electron microscope, many single spherical buds were observed on the surface of H3/Osaka virus-infected cells incubated without GS4071, whereas many aggregated particles were observed on the surface of cells incubated with GS4071. However, many long tubular virus-like structures, with no aggregated particles, were observed on the surface of H10/chicken virus-infected cells incubated with GS4071. The same results were obtained when another NA inhibitor, zanamivir, was used. CONCLUSIONS: These results indicate that NA inhibitors interfered with virus particle formation in the H10/chicken virus-infected cells, in which the inhibitor caused the formation of long tubular virus-like structures instead of spherical virus particles. BioMed Central 2011-08-19 /pmc/articles/PMC3170304/ /pubmed/21851647 http://dx.doi.org/10.1186/1743-422X-8-411 Text en Copyright ©2011 Ushirogawa and Ohuchi; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ushirogawa, Hiroshi
Ohuchi, Masanobu
Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title_full Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title_fullStr Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title_full_unstemmed Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title_short Novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
title_sort novel antiviral activity of neuraminidase inhibitors against an avian influenza a virus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170304/
https://www.ncbi.nlm.nih.gov/pubmed/21851647
http://dx.doi.org/10.1186/1743-422X-8-411
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