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Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats

BACKGROUND: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development. METHODS: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical sm...

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Autores principales: Damasceno, Débora C, Sinzato, Yuri K, Lima, Paula H, de Souza, Maricelma S, Campos, Kleber E, Dallaqua, Bruna, Calderon, Iracema M, Rudge, Marilza V, Volpato, Gustavo T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170573/
https://www.ncbi.nlm.nih.gov/pubmed/21851636
http://dx.doi.org/10.1186/1758-5996-3-20
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author Damasceno, Débora C
Sinzato, Yuri K
Lima, Paula H
de Souza, Maricelma S
Campos, Kleber E
Dallaqua, Bruna
Calderon, Iracema M
Rudge, Marilza V
Volpato, Gustavo T
author_facet Damasceno, Débora C
Sinzato, Yuri K
Lima, Paula H
de Souza, Maricelma S
Campos, Kleber E
Dallaqua, Bruna
Calderon, Iracema M
Rudge, Marilza V
Volpato, Gustavo T
author_sort Damasceno, Débora C
collection PubMed
description BACKGROUND: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development. METHODS: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes. RESULTS: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals. CONCLUSION: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development.
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spelling pubmed-31705732011-09-11 Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats Damasceno, Débora C Sinzato, Yuri K Lima, Paula H de Souza, Maricelma S Campos, Kleber E Dallaqua, Bruna Calderon, Iracema M Rudge, Marilza V Volpato, Gustavo T Diabetol Metab Syndr Research BACKGROUND: The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development. METHODS: Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female Wistar rats were randomly distributed in four experimental groups (n minimum = 13/group): nondiabetic (ND) and diabetic rats exposed to filtered air (D), diabetic rats exposed to cigarette smoke prior to and into the pregnancy period (DS) and diabetic rats exposed to cigarette smoke prior to pregnancy period (DSPP). At day 21 of pregnancy, rats were killed for maternal biochemical determination and reproductive outcomes. RESULTS: The association of diabetes and cigarette smoke in DSPP group caused altered glycemia at term, reduced number of implantation and live fetuses, decreased litter and maternal weight, increased pre and postimplantation loss rates, reduced triglyceride and VLDL-c concentrations, increased levels of thiol groups and MDA. Besides, these dams presented increased SOD and GSH-Px activities. However, the increased antioxidant status was not sufficient to prevent the lipid peroxidation observed in these animals. CONCLUSION: Despite the benefits stemming from smoking interruption during the pregnancy of diabetic rats, such improvement was insufficient to avoid metabolic alterations and provide an adequate intrauterine environment for embryofetal development. Therefore, these results suggest that it is necessary to cease smoking extensive time before planning pregnancy, since stopping smoking only when pregnancy is detected may not contribute effectively to fully adequate embryofetal development. BioMed Central 2011-08-18 /pmc/articles/PMC3170573/ /pubmed/21851636 http://dx.doi.org/10.1186/1758-5996-3-20 Text en Copyright ©2011 Damasceno et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Damasceno, Débora C
Sinzato, Yuri K
Lima, Paula H
de Souza, Maricelma S
Campos, Kleber E
Dallaqua, Bruna
Calderon, Iracema M
Rudge, Marilza V
Volpato, Gustavo T
Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_full Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_fullStr Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_full_unstemmed Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_short Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
title_sort effects of exposure to cigarette smoke prior to pregnancy in diabetic rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170573/
https://www.ncbi.nlm.nih.gov/pubmed/21851636
http://dx.doi.org/10.1186/1758-5996-3-20
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