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CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals
A proportional balance between αβ and γδ T cell subsets in the periphery is exceedingly well maintained via a homeostatic mechanism. However, a cellular mechanism underlying the regulation remains undefined. We recently reported that a subset of developing γδ T cells spontaneously acquire IL-17-prod...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170686/ https://www.ncbi.nlm.nih.gov/pubmed/21647171 http://dx.doi.org/10.1038/icb.2011.50 |
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author | Do, Jeong-Su Visperas, Anabelle O’Brien, Rebecca L. Min, Booki |
author_facet | Do, Jeong-Su Visperas, Anabelle O’Brien, Rebecca L. Min, Booki |
author_sort | Do, Jeong-Su |
collection | PubMed |
description | A proportional balance between αβ and γδ T cell subsets in the periphery is exceedingly well maintained via a homeostatic mechanism. However, a cellular mechanism underlying the regulation remains undefined. We recently reported that a subset of developing γδ T cells spontaneously acquire IL-17-producing capacity even within naïve animals via a TGFβ1-dependent mechanism, thus considered ‘einnate’ IL-17-producing cells. Here we report that γδ T cells generated within αβ T cell (or CD4 T cell)-deficient environments displayed altered cytokine profiles; particularly, ‘einnate’ IL-17 expression was significantly impaired compared to those in wild type mice. Impaired IL-17 production in γδ T cells was directly related to the CD4 T cell deficiency, because depletion of CD4 T cells in wild type mice diminished and adoptive CD4 T cell transfer into TCRβ−/− mice restored IL-17 expression in γδ T cells. CD4 T cell-mediated IL-17 expression required TGFβ1. Moreover, Th17 but not Th1 or Th2 effector CD4 T cells were highly efficient in enhancing γδ T cell IL-17 expression. Taken together, our results highlight a novel CD4 T cell-dependent mechanism that shapes the generation of IL-17+ γδ T cells in naïve settings. |
format | Online Article Text |
id | pubmed-3170686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-31706862012-10-01 CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals Do, Jeong-Su Visperas, Anabelle O’Brien, Rebecca L. Min, Booki Immunol Cell Biol Article A proportional balance between αβ and γδ T cell subsets in the periphery is exceedingly well maintained via a homeostatic mechanism. However, a cellular mechanism underlying the regulation remains undefined. We recently reported that a subset of developing γδ T cells spontaneously acquire IL-17-producing capacity even within naïve animals via a TGFβ1-dependent mechanism, thus considered ‘einnate’ IL-17-producing cells. Here we report that γδ T cells generated within αβ T cell (or CD4 T cell)-deficient environments displayed altered cytokine profiles; particularly, ‘einnate’ IL-17 expression was significantly impaired compared to those in wild type mice. Impaired IL-17 production in γδ T cells was directly related to the CD4 T cell deficiency, because depletion of CD4 T cells in wild type mice diminished and adoptive CD4 T cell transfer into TCRβ−/− mice restored IL-17 expression in γδ T cells. CD4 T cell-mediated IL-17 expression required TGFβ1. Moreover, Th17 but not Th1 or Th2 effector CD4 T cells were highly efficient in enhancing γδ T cell IL-17 expression. Taken together, our results highlight a novel CD4 T cell-dependent mechanism that shapes the generation of IL-17+ γδ T cells in naïve settings. 2011-06-07 2012-04 /pmc/articles/PMC3170686/ /pubmed/21647171 http://dx.doi.org/10.1038/icb.2011.50 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Do, Jeong-Su Visperas, Anabelle O’Brien, Rebecca L. Min, Booki CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title | CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title_full | CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title_fullStr | CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title_full_unstemmed | CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title_short | CD4 T cells play important roles in maintaining IL-17-producing γδ T cell subsets in naïve animals |
title_sort | cd4 t cells play important roles in maintaining il-17-producing γδ t cell subsets in naïve animals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170686/ https://www.ncbi.nlm.nih.gov/pubmed/21647171 http://dx.doi.org/10.1038/icb.2011.50 |
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