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Screening of Supports for the Immobilization of β-Glucosidase
A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170910/ https://www.ncbi.nlm.nih.gov/pubmed/21915374 http://dx.doi.org/10.4061/2011/642460 |
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author | Figueira, Joelise de Alencar Dias, Fernanda Furlan Gonçalves Sato, Hélia Harumi Fernandes, Pedro |
author_facet | Figueira, Joelise de Alencar Dias, Fernanda Furlan Gonçalves Sato, Hélia Harumi Fernandes, Pedro |
author_sort | Figueira, Joelise de Alencar |
collection | PubMed |
description | A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization. Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized. Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed. Both thermal and pH stability were improved as a result of immobilization. An increase in the apparent K (M) (Michaelis constant) was observed following immobilization, suggesting diffusion limitations. |
format | Online Article Text |
id | pubmed-3170910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-31709102011-09-13 Screening of Supports for the Immobilization of β-Glucosidase Figueira, Joelise de Alencar Dias, Fernanda Furlan Gonçalves Sato, Hélia Harumi Fernandes, Pedro Enzyme Res Research Article A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization. Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized. Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed. Both thermal and pH stability were improved as a result of immobilization. An increase in the apparent K (M) (Michaelis constant) was observed following immobilization, suggesting diffusion limitations. SAGE-Hindawi Access to Research 2011 2011-09-11 /pmc/articles/PMC3170910/ /pubmed/21915374 http://dx.doi.org/10.4061/2011/642460 Text en Copyright © 2011 Joelise de Alencar Figueira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Figueira, Joelise de Alencar Dias, Fernanda Furlan Gonçalves Sato, Hélia Harumi Fernandes, Pedro Screening of Supports for the Immobilization of β-Glucosidase |
title | Screening of Supports for the Immobilization of β-Glucosidase |
title_full | Screening of Supports for the Immobilization of β-Glucosidase |
title_fullStr | Screening of Supports for the Immobilization of β-Glucosidase |
title_full_unstemmed | Screening of Supports for the Immobilization of β-Glucosidase |
title_short | Screening of Supports for the Immobilization of β-Glucosidase |
title_sort | screening of supports for the immobilization of β-glucosidase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170910/ https://www.ncbi.nlm.nih.gov/pubmed/21915374 http://dx.doi.org/10.4061/2011/642460 |
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