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Screening of Supports for the Immobilization of β-Glucosidase

A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation...

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Autores principales: Figueira, Joelise de Alencar, Dias, Fernanda Furlan Gonçalves, Sato, Hélia Harumi, Fernandes, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170910/
https://www.ncbi.nlm.nih.gov/pubmed/21915374
http://dx.doi.org/10.4061/2011/642460
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author Figueira, Joelise de Alencar
Dias, Fernanda Furlan Gonçalves
Sato, Hélia Harumi
Fernandes, Pedro
author_facet Figueira, Joelise de Alencar
Dias, Fernanda Furlan Gonçalves
Sato, Hélia Harumi
Fernandes, Pedro
author_sort Figueira, Joelise de Alencar
collection PubMed
description A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization. Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized. Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed. Both thermal and pH stability were improved as a result of immobilization. An increase in the apparent K (M) (Michaelis constant) was observed following immobilization, suggesting diffusion limitations.
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spelling pubmed-31709102011-09-13 Screening of Supports for the Immobilization of β-Glucosidase Figueira, Joelise de Alencar Dias, Fernanda Furlan Gonçalves Sato, Hélia Harumi Fernandes, Pedro Enzyme Res Research Article A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization. Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized. Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed. Both thermal and pH stability were improved as a result of immobilization. An increase in the apparent K (M) (Michaelis constant) was observed following immobilization, suggesting diffusion limitations. SAGE-Hindawi Access to Research 2011 2011-09-11 /pmc/articles/PMC3170910/ /pubmed/21915374 http://dx.doi.org/10.4061/2011/642460 Text en Copyright © 2011 Joelise de Alencar Figueira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Figueira, Joelise de Alencar
Dias, Fernanda Furlan Gonçalves
Sato, Hélia Harumi
Fernandes, Pedro
Screening of Supports for the Immobilization of β-Glucosidase
title Screening of Supports for the Immobilization of β-Glucosidase
title_full Screening of Supports for the Immobilization of β-Glucosidase
title_fullStr Screening of Supports for the Immobilization of β-Glucosidase
title_full_unstemmed Screening of Supports for the Immobilization of β-Glucosidase
title_short Screening of Supports for the Immobilization of β-Glucosidase
title_sort screening of supports for the immobilization of β-glucosidase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170910/
https://www.ncbi.nlm.nih.gov/pubmed/21915374
http://dx.doi.org/10.4061/2011/642460
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