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CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen
Cells undergoing programmed cell death (apoptosis) are removed in situ by macrophages and dendritic cells (DCs) through a specialized form of phagocytosis (efferocytosis). In the lung, there are two primary DC subsets with the potential to migrate to the local lymph nodes (LNs) and initiate adaptive...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171085/ https://www.ncbi.nlm.nih.gov/pubmed/21859845 http://dx.doi.org/10.1084/jem.20110538 |
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author | Desch, A. Nicole Randolph, Gwendalyn J. Murphy, Kenneth Gautier, Emmanuel L. Kedl, Ross M. Lahoud, Mireille H. Caminschi, Irina Shortman, Ken Henson, Peter M. Jakubzick, Claudia V. |
author_facet | Desch, A. Nicole Randolph, Gwendalyn J. Murphy, Kenneth Gautier, Emmanuel L. Kedl, Ross M. Lahoud, Mireille H. Caminschi, Irina Shortman, Ken Henson, Peter M. Jakubzick, Claudia V. |
author_sort | Desch, A. Nicole |
collection | PubMed |
description | Cells undergoing programmed cell death (apoptosis) are removed in situ by macrophages and dendritic cells (DCs) through a specialized form of phagocytosis (efferocytosis). In the lung, there are two primary DC subsets with the potential to migrate to the local lymph nodes (LNs) and initiate adaptive immune responses. In this study, we show that only CD103(+) DCs were able to acquire and transport apoptotic cells to the draining LNs and cross present apoptotic cell–associated antigen to CD8 T cells. In contrast, both the CD11b(hi) and the CD103(+) DCs were able to ingest and traffic latex beads or soluble antigen. CD103(+) DCs selectively exhibited high expression of TLR3, and ligation of this receptor led to enhanced in vivo cytotoxic T cell responses to apoptotic cell–associated antigen. The selective role for CD103(+) DCs was confirmed in Batf3(−/−) mice, which lack this DC subtype. Our findings suggest that CD103(+) DCs are the DC subset in the lung that captures and presents apoptotic cell–associated antigen under homeostatic and inflammatory conditions and raise the possibility for more focused immunological targeting to CD8 T cell responses. |
format | Online Article Text |
id | pubmed-3171085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31710852012-02-29 CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen Desch, A. Nicole Randolph, Gwendalyn J. Murphy, Kenneth Gautier, Emmanuel L. Kedl, Ross M. Lahoud, Mireille H. Caminschi, Irina Shortman, Ken Henson, Peter M. Jakubzick, Claudia V. J Exp Med Brief Definitive Report Cells undergoing programmed cell death (apoptosis) are removed in situ by macrophages and dendritic cells (DCs) through a specialized form of phagocytosis (efferocytosis). In the lung, there are two primary DC subsets with the potential to migrate to the local lymph nodes (LNs) and initiate adaptive immune responses. In this study, we show that only CD103(+) DCs were able to acquire and transport apoptotic cells to the draining LNs and cross present apoptotic cell–associated antigen to CD8 T cells. In contrast, both the CD11b(hi) and the CD103(+) DCs were able to ingest and traffic latex beads or soluble antigen. CD103(+) DCs selectively exhibited high expression of TLR3, and ligation of this receptor led to enhanced in vivo cytotoxic T cell responses to apoptotic cell–associated antigen. The selective role for CD103(+) DCs was confirmed in Batf3(−/−) mice, which lack this DC subtype. Our findings suggest that CD103(+) DCs are the DC subset in the lung that captures and presents apoptotic cell–associated antigen under homeostatic and inflammatory conditions and raise the possibility for more focused immunological targeting to CD8 T cell responses. The Rockefeller University Press 2011-08-29 /pmc/articles/PMC3171085/ /pubmed/21859845 http://dx.doi.org/10.1084/jem.20110538 Text en © 2011 Desch et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Brief Definitive Report Desch, A. Nicole Randolph, Gwendalyn J. Murphy, Kenneth Gautier, Emmanuel L. Kedl, Ross M. Lahoud, Mireille H. Caminschi, Irina Shortman, Ken Henson, Peter M. Jakubzick, Claudia V. CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title | CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title_full | CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title_fullStr | CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title_full_unstemmed | CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title_short | CD103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
title_sort | cd103(+) pulmonary dendritic cells preferentially acquire and present apoptotic cell–associated antigen |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171085/ https://www.ncbi.nlm.nih.gov/pubmed/21859845 http://dx.doi.org/10.1084/jem.20110538 |
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