Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons

The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments wer...

Descripción completa

Detalles Bibliográficos
Autores principales: Lueneberg, Kathia, Domínguez, Guadalupe, Arias-Carrión, Oscar, Palomero-Rivero, Marcela, Millán-Aldaco, Diana, Morán, Julio, Drucker-Colín, René, Murillo-Rodríguez, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171300/
https://www.ncbi.nlm.nih.gov/pubmed/21854612
http://dx.doi.org/10.1186/1755-7682-4-28
Descripción
Sumario:The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.

Ejemplares similares