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Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons
The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments wer...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171300/ https://www.ncbi.nlm.nih.gov/pubmed/21854612 http://dx.doi.org/10.1186/1755-7682-4-28 |
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author | Lueneberg, Kathia Domínguez, Guadalupe Arias-Carrión, Oscar Palomero-Rivero, Marcela Millán-Aldaco, Diana Morán, Julio Drucker-Colín, René Murillo-Rodríguez, Eric |
author_facet | Lueneberg, Kathia Domínguez, Guadalupe Arias-Carrión, Oscar Palomero-Rivero, Marcela Millán-Aldaco, Diana Morán, Julio Drucker-Colín, René Murillo-Rodríguez, Eric |
author_sort | Lueneberg, Kathia |
collection | PubMed |
description | The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death. |
format | Online Article Text |
id | pubmed-3171300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31713002011-09-13 Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons Lueneberg, Kathia Domínguez, Guadalupe Arias-Carrión, Oscar Palomero-Rivero, Marcela Millán-Aldaco, Diana Morán, Julio Drucker-Colín, René Murillo-Rodríguez, Eric Int Arch Med Review The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death. BioMed Central 2011-08-19 /pmc/articles/PMC3171300/ /pubmed/21854612 http://dx.doi.org/10.1186/1755-7682-4-28 Text en Copyright ©2011 Lueneberg et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Lueneberg, Kathia Domínguez, Guadalupe Arias-Carrión, Oscar Palomero-Rivero, Marcela Millán-Aldaco, Diana Morán, Julio Drucker-Colín, René Murillo-Rodríguez, Eric Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title | Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title_full | Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title_fullStr | Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title_full_unstemmed | Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title_short | Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
title_sort | cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171300/ https://www.ncbi.nlm.nih.gov/pubmed/21854612 http://dx.doi.org/10.1186/1755-7682-4-28 |
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