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Real-time PCR detection of Plasmodium directly from whole blood and filter paper samples

BACKGROUND: Real-time PCR is a sensitive and specific method for the analysis of Plasmodium DNA. However, prior purification of genomic DNA from blood is necessary since PCR inhibitors and quenching of fluorophores from blood prevent efficient amplification and detection of PCR products. METHODS: Re...

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Detalles Bibliográficos
Autores principales:	Taylor, Brian J, Martin, Kimberly A, Arango, Eliana, Agudelo, Olga M, Maestre, Amanda, Yanow, Stephanie K
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171379/ https://www.ncbi.nlm.nih.gov/pubmed/21851640 http://dx.doi.org/10.1186/1475-2875-10-244

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