Cargando…
Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration
BACKGROUND: CXCL12 is a pleiotropic chemokine involved in multiple different processes such as immune regulation, inflammatory responses, and cancer development. CXCL12 is also a potent chemokine involved in chemoattraction of T cells to the site of infection or inflammation. Mammalian target of rap...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171460/ https://www.ncbi.nlm.nih.gov/pubmed/21931802 http://dx.doi.org/10.1371/journal.pone.0024667 |
_version_ | 1782211767048339456 |
---|---|
author | Munk, Rachel Ghosh, Paritosh Ghosh, Manik C. Saito, Takeshi Xu, Mai Carter, Arnell Indig, Fred Taub, Dennis D. Longo, Dan L. |
author_facet | Munk, Rachel Ghosh, Paritosh Ghosh, Manik C. Saito, Takeshi Xu, Mai Carter, Arnell Indig, Fred Taub, Dennis D. Longo, Dan L. |
author_sort | Munk, Rachel |
collection | PubMed |
description | BACKGROUND: CXCL12 is a pleiotropic chemokine involved in multiple different processes such as immune regulation, inflammatory responses, and cancer development. CXCL12 is also a potent chemokine involved in chemoattraction of T cells to the site of infection or inflammation. Mammalian target of rapamycin (mTOR) is a serine-threonine kinase that modulates different cellular processes, such as metabolism, nutrient sensing, protein translation, and cell growth. The role of mTOR in CXCL12-mediated resting T cell migration has yet to be elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Rapamycin, an inhibitor of mTOR, significantly inhibits CXCL12 mediated migration of both primary human resting T cells and human T cell leukemia cell line CEM. p70(S6K1), an effector molecule of mTOR signaling pathway, was knocked down by shRNA in CEM cells using a lentiviral gene transfer system. Using p70(S6K1) knock down cells, we demonstrate the role of mTOR signaling in T cell migration both in vitro and in vivo. CONCLUSIONS: Our data demonstrate a new role for mTOR in CXCL12-induced T cell migration, and enrich the current knowledge regarding the clinical use of rapamycin. |
format | Online Article Text |
id | pubmed-3171460 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31714602011-09-19 Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration Munk, Rachel Ghosh, Paritosh Ghosh, Manik C. Saito, Takeshi Xu, Mai Carter, Arnell Indig, Fred Taub, Dennis D. Longo, Dan L. PLoS One Research Article BACKGROUND: CXCL12 is a pleiotropic chemokine involved in multiple different processes such as immune regulation, inflammatory responses, and cancer development. CXCL12 is also a potent chemokine involved in chemoattraction of T cells to the site of infection or inflammation. Mammalian target of rapamycin (mTOR) is a serine-threonine kinase that modulates different cellular processes, such as metabolism, nutrient sensing, protein translation, and cell growth. The role of mTOR in CXCL12-mediated resting T cell migration has yet to be elucidated. METHODOLOGY/PRINCIPAL FINDINGS: Rapamycin, an inhibitor of mTOR, significantly inhibits CXCL12 mediated migration of both primary human resting T cells and human T cell leukemia cell line CEM. p70(S6K1), an effector molecule of mTOR signaling pathway, was knocked down by shRNA in CEM cells using a lentiviral gene transfer system. Using p70(S6K1) knock down cells, we demonstrate the role of mTOR signaling in T cell migration both in vitro and in vivo. CONCLUSIONS: Our data demonstrate a new role for mTOR in CXCL12-induced T cell migration, and enrich the current knowledge regarding the clinical use of rapamycin. Public Library of Science 2011-09-12 /pmc/articles/PMC3171460/ /pubmed/21931802 http://dx.doi.org/10.1371/journal.pone.0024667 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Munk, Rachel Ghosh, Paritosh Ghosh, Manik C. Saito, Takeshi Xu, Mai Carter, Arnell Indig, Fred Taub, Dennis D. Longo, Dan L. Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title | Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title_full | Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title_fullStr | Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title_full_unstemmed | Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title_short | Involvement of mTOR in CXCL12 Mediated T Cell Signaling and Migration |
title_sort | involvement of mtor in cxcl12 mediated t cell signaling and migration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171460/ https://www.ncbi.nlm.nih.gov/pubmed/21931802 http://dx.doi.org/10.1371/journal.pone.0024667 |
work_keys_str_mv | AT munkrachel involvementofmtorincxcl12mediatedtcellsignalingandmigration AT ghoshparitosh involvementofmtorincxcl12mediatedtcellsignalingandmigration AT ghoshmanikc involvementofmtorincxcl12mediatedtcellsignalingandmigration AT saitotakeshi involvementofmtorincxcl12mediatedtcellsignalingandmigration AT xumai involvementofmtorincxcl12mediatedtcellsignalingandmigration AT carterarnell involvementofmtorincxcl12mediatedtcellsignalingandmigration AT indigfred involvementofmtorincxcl12mediatedtcellsignalingandmigration AT taubdennisd involvementofmtorincxcl12mediatedtcellsignalingandmigration AT longodanl involvementofmtorincxcl12mediatedtcellsignalingandmigration |