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Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison
The advent of X-ray free-electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program req...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171899/ https://www.ncbi.nlm.nih.gov/pubmed/21844653 http://dx.doi.org/10.1107/S0108767311019611 |
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author | Moths, Brian Ourmazd, Abbas |
author_facet | Moths, Brian Ourmazd, Abbas |
author_sort | Moths, Brian |
collection | PubMed |
description | The advent of X-ray free-electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program requires the ability to determine the orientation of the particle giving rise to each snapshot at signal levels as low as ~10(−2) photons per pixel. Two apparently different approaches have recently demonstrated this capability. Here we show they represent different implementations of the same fundamental approach, and identify the primary factors limiting their performance. |
format | Online Article Text |
id | pubmed-3171899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-31718992011-09-14 Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison Moths, Brian Ourmazd, Abbas Acta Crystallogr A Research Papers The advent of X-ray free-electron lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is destroyed by the intense femtosecond pulse. This program requires the ability to determine the orientation of the particle giving rise to each snapshot at signal levels as low as ~10(−2) photons per pixel. Two apparently different approaches have recently demonstrated this capability. Here we show they represent different implementations of the same fundamental approach, and identify the primary factors limiting their performance. International Union of Crystallography 2011-09-01 2011-07-06 /pmc/articles/PMC3171899/ /pubmed/21844653 http://dx.doi.org/10.1107/S0108767311019611 Text en © Moths and Ourmazd 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Moths, Brian Ourmazd, Abbas Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title | Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title_full | Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title_fullStr | Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title_full_unstemmed | Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title_short | Bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
title_sort | bayesian algorithms for recovering structure from single-particle diffraction snapshots of unknown orientation: a comparison |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3171899/ https://www.ncbi.nlm.nih.gov/pubmed/21844653 http://dx.doi.org/10.1107/S0108767311019611 |
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