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Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium

Prokineticin 1 (PROK1) signalling via prokineticin receptor 1 (PROKR1) regulates the expression of several genes with important roles in endometrial receptivity and implantation. This study investigated PROK1 regulation of Dickkopf 1 (DKK1) expression, a negative regulator of canonical Wnt signallin...

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Autores principales: Macdonald, Linsay J., Sales, Kurt J., Grant, Vivien, Brown, Pamela, Jabbour, Henry N., Catalano, Rob D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172036/
https://www.ncbi.nlm.nih.gov/pubmed/21546446
http://dx.doi.org/10.1093/molehr/gar031
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author Macdonald, Linsay J.
Sales, Kurt J.
Grant, Vivien
Brown, Pamela
Jabbour, Henry N.
Catalano, Rob D.
author_facet Macdonald, Linsay J.
Sales, Kurt J.
Grant, Vivien
Brown, Pamela
Jabbour, Henry N.
Catalano, Rob D.
author_sort Macdonald, Linsay J.
collection PubMed
description Prokineticin 1 (PROK1) signalling via prokineticin receptor 1 (PROKR1) regulates the expression of several genes with important roles in endometrial receptivity and implantation. This study investigated PROK1 regulation of Dickkopf 1 (DKK1) expression, a negative regulator of canonical Wnt signalling, and its function in the non-pregnant endometrium and first trimester decidua. DKK1 mRNA expression is elevated during the mid-secretory phase of the menstrual cycle and expression increases further in first trimester decidua. DKK1 protein expression is localized to glandular epithelial and stromal cells during the proliferative, early- and mid-secretory phases, whereas expression is confined to the stroma in the late-secretory phase and first trimester decidua. PROK1 induces the expression of DKK1 in endometrial epithelial cells stably expressing PROKR1 and in first trimester decidua explants, via a Gq-calcium-calcineurin-nuclear factor of activated T-cells-mediated pathway. Endometrial epithelial cell proliferation is negatively regulated by PROK1-PROKR1 signalling. We demonstrate that this effect on cell proliferation occurs via DKK1 expression, as siRNA targeted against DKK1 reduces the PROK1-induced decrease in proliferation. Furthermore, decidualization of primary human endometrial stromal cells with progesterone and cyclic adenosine monophosphate is inhibited by miRNA knock down of PROK1 or DKK1. These data demonstrate important roles for PROK1 and DKK1 during endometrial receptivity and early pregnancy, which include regulation of endometrial cell proliferation and decidualization.
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spelling pubmed-31720362011-09-13 Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium Macdonald, Linsay J. Sales, Kurt J. Grant, Vivien Brown, Pamela Jabbour, Henry N. Catalano, Rob D. Mol Hum Reprod Articles Prokineticin 1 (PROK1) signalling via prokineticin receptor 1 (PROKR1) regulates the expression of several genes with important roles in endometrial receptivity and implantation. This study investigated PROK1 regulation of Dickkopf 1 (DKK1) expression, a negative regulator of canonical Wnt signalling, and its function in the non-pregnant endometrium and first trimester decidua. DKK1 mRNA expression is elevated during the mid-secretory phase of the menstrual cycle and expression increases further in first trimester decidua. DKK1 protein expression is localized to glandular epithelial and stromal cells during the proliferative, early- and mid-secretory phases, whereas expression is confined to the stroma in the late-secretory phase and first trimester decidua. PROK1 induces the expression of DKK1 in endometrial epithelial cells stably expressing PROKR1 and in first trimester decidua explants, via a Gq-calcium-calcineurin-nuclear factor of activated T-cells-mediated pathway. Endometrial epithelial cell proliferation is negatively regulated by PROK1-PROKR1 signalling. We demonstrate that this effect on cell proliferation occurs via DKK1 expression, as siRNA targeted against DKK1 reduces the PROK1-induced decrease in proliferation. Furthermore, decidualization of primary human endometrial stromal cells with progesterone and cyclic adenosine monophosphate is inhibited by miRNA knock down of PROK1 or DKK1. These data demonstrate important roles for PROK1 and DKK1 during endometrial receptivity and early pregnancy, which include regulation of endometrial cell proliferation and decidualization. Oxford University Press 2011-10 2011-05-05 /pmc/articles/PMC3172036/ /pubmed/21546446 http://dx.doi.org/10.1093/molehr/gar031 Text en © The Author 2011. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology http://creativecommons.org/licenses/by-nc/2.5/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Macdonald, Linsay J.
Sales, Kurt J.
Grant, Vivien
Brown, Pamela
Jabbour, Henry N.
Catalano, Rob D.
Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title_full Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title_fullStr Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title_full_unstemmed Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title_short Prokineticin 1 induces Dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
title_sort prokineticin 1 induces dickkopf 1 expression and regulates cell proliferation and decidualization in the human endometrium
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172036/
https://www.ncbi.nlm.nih.gov/pubmed/21546446
http://dx.doi.org/10.1093/molehr/gar031
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