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N-WASP is required for membrane wrapping and myelination by Schwann cells
During peripheral nerve myelination, Schwann cells sort larger axons, ensheath them, and eventually wrap their membrane to form the myelin sheath. These processes involve extensive changes in cell shape, but the exact mechanisms involved are still unknown. Neural Wiskott–Aldrich syndrome protein (N-...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172181/ https://www.ncbi.nlm.nih.gov/pubmed/21263026 http://dx.doi.org/10.1083/jcb.201010013 |
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author | Novak, Nurit Bar, Vered Sabanay, Helena Frechter, Shahar Jaegle, Martine Snapper, Scott B. Meijer, Dies Peles, Elior |
author_facet | Novak, Nurit Bar, Vered Sabanay, Helena Frechter, Shahar Jaegle, Martine Snapper, Scott B. Meijer, Dies Peles, Elior |
author_sort | Novak, Nurit |
collection | PubMed |
description | During peripheral nerve myelination, Schwann cells sort larger axons, ensheath them, and eventually wrap their membrane to form the myelin sheath. These processes involve extensive changes in cell shape, but the exact mechanisms involved are still unknown. Neural Wiskott–Aldrich syndrome protein (N-WASP) integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the Arp2/3 complex. By generating mice lacking N-WASP in myelinating Schwann cells, we show that N-WASP is crucial for myelination. In N-WASP–deficient nerves, Schwann cells sort and ensheath axons, but most of them fail to myelinate and arrest at the promyelinating stage. Yet, a limited number of Schwann cells form unusually short internodes, containing thin myelin sheaths, with the occasional appearance of myelin misfoldings. These data suggest that regulation of actin filament nucleation in Schwann cells by N-WASP is crucial for membrane wrapping, longitudinal extension, and myelination. |
format | Online Article Text |
id | pubmed-3172181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31721812011-09-14 N-WASP is required for membrane wrapping and myelination by Schwann cells Novak, Nurit Bar, Vered Sabanay, Helena Frechter, Shahar Jaegle, Martine Snapper, Scott B. Meijer, Dies Peles, Elior J Cell Biol Research Articles During peripheral nerve myelination, Schwann cells sort larger axons, ensheath them, and eventually wrap their membrane to form the myelin sheath. These processes involve extensive changes in cell shape, but the exact mechanisms involved are still unknown. Neural Wiskott–Aldrich syndrome protein (N-WASP) integrates various extracellular signals to control actin dynamics and cytoskeletal reorganization through activation of the Arp2/3 complex. By generating mice lacking N-WASP in myelinating Schwann cells, we show that N-WASP is crucial for myelination. In N-WASP–deficient nerves, Schwann cells sort and ensheath axons, but most of them fail to myelinate and arrest at the promyelinating stage. Yet, a limited number of Schwann cells form unusually short internodes, containing thin myelin sheaths, with the occasional appearance of myelin misfoldings. These data suggest that regulation of actin filament nucleation in Schwann cells by N-WASP is crucial for membrane wrapping, longitudinal extension, and myelination. The Rockefeller University Press 2011-01-24 /pmc/articles/PMC3172181/ /pubmed/21263026 http://dx.doi.org/10.1083/jcb.201010013 Text en © 2011 Novak et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Novak, Nurit Bar, Vered Sabanay, Helena Frechter, Shahar Jaegle, Martine Snapper, Scott B. Meijer, Dies Peles, Elior N-WASP is required for membrane wrapping and myelination by Schwann cells |
title | N-WASP is required for membrane wrapping and myelination by Schwann cells |
title_full | N-WASP is required for membrane wrapping and myelination by Schwann cells |
title_fullStr | N-WASP is required for membrane wrapping and myelination by Schwann cells |
title_full_unstemmed | N-WASP is required for membrane wrapping and myelination by Schwann cells |
title_short | N-WASP is required for membrane wrapping and myelination by Schwann cells |
title_sort | n-wasp is required for membrane wrapping and myelination by schwann cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172181/ https://www.ncbi.nlm.nih.gov/pubmed/21263026 http://dx.doi.org/10.1083/jcb.201010013 |
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