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Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes

In many eukaryotes, condensins I and II associate with chromosomes in an ordered fashion during mitosis and play nonoverlapping functions in their assembly and segregation. Here we report for the first time the spatiotemporal dynamics and functions of the two condensin complexes during meiotic divis...

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Autores principales: Lee, Jibak, Ogushi, Sugako, Saitou, Mitinori, Hirano, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172270/
https://www.ncbi.nlm.nih.gov/pubmed/21795393
http://dx.doi.org/10.1091/mbc.E11-05-0423
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author Lee, Jibak
Ogushi, Sugako
Saitou, Mitinori
Hirano, Tatsuya
author_facet Lee, Jibak
Ogushi, Sugako
Saitou, Mitinori
Hirano, Tatsuya
author_sort Lee, Jibak
collection PubMed
description In many eukaryotes, condensins I and II associate with chromosomes in an ordered fashion during mitosis and play nonoverlapping functions in their assembly and segregation. Here we report for the first time the spatiotemporal dynamics and functions of the two condensin complexes during meiotic divisions in mouse oocytes. At the germinal vesicle stage (prophase I), condensin I is present in the cytoplasm, whereas condensin II is localized within the nucleus. After germinal vesicle breakdown, condensin II starts to associate with chromosomes and becomes concentrated onto chromatid axes of bivalent chromosomes by metaphase I. REC8 “glues” chromosome arms along their lengths. In striking contrast to condensin II, condensin I localizes primarily around centromeric regions at metaphase I and starts to associate stably with chromosome arms only after anaphase I. Antibody injection experiments show that condensin functions are required for many aspects of meiotic chromosome dynamics, including chromosome individualization, resolution, and segregation. We propose that the two condensin complexes play distinctive roles in constructing bivalent chromosomes: condensin II might play a primary role in resolving sister chromatid axes, whereas condensin I might contribute to monopolar attachment of sister kinetochores, possibly by assembling a unique centromeric structure underneath.
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spelling pubmed-31722702011-11-30 Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes Lee, Jibak Ogushi, Sugako Saitou, Mitinori Hirano, Tatsuya Mol Biol Cell Articles In many eukaryotes, condensins I and II associate with chromosomes in an ordered fashion during mitosis and play nonoverlapping functions in their assembly and segregation. Here we report for the first time the spatiotemporal dynamics and functions of the two condensin complexes during meiotic divisions in mouse oocytes. At the germinal vesicle stage (prophase I), condensin I is present in the cytoplasm, whereas condensin II is localized within the nucleus. After germinal vesicle breakdown, condensin II starts to associate with chromosomes and becomes concentrated onto chromatid axes of bivalent chromosomes by metaphase I. REC8 “glues” chromosome arms along their lengths. In striking contrast to condensin II, condensin I localizes primarily around centromeric regions at metaphase I and starts to associate stably with chromosome arms only after anaphase I. Antibody injection experiments show that condensin functions are required for many aspects of meiotic chromosome dynamics, including chromosome individualization, resolution, and segregation. We propose that the two condensin complexes play distinctive roles in constructing bivalent chromosomes: condensin II might play a primary role in resolving sister chromatid axes, whereas condensin I might contribute to monopolar attachment of sister kinetochores, possibly by assembling a unique centromeric structure underneath. The American Society for Cell Biology 2011-09-15 /pmc/articles/PMC3172270/ /pubmed/21795393 http://dx.doi.org/10.1091/mbc.E11-05-0423 Text en © 2011 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Lee, Jibak
Ogushi, Sugako
Saitou, Mitinori
Hirano, Tatsuya
Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title_full Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title_fullStr Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title_full_unstemmed Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title_short Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes
title_sort condensins i and ii are essential for construction of bivalent chromosomes in mouse oocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172270/
https://www.ncbi.nlm.nih.gov/pubmed/21795393
http://dx.doi.org/10.1091/mbc.E11-05-0423
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