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The period of the somite segmentation clock is sensitive to Notch activity
The number of vertebrae is defined strictly for a given species and depends on the number of somites, which are the earliest metameric structures that form in development. Somites are formed by sequential segmentation. The periodicity of somite segmentation is orchestrated by the synchronous oscilla...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172277/ https://www.ncbi.nlm.nih.gov/pubmed/21795391 http://dx.doi.org/10.1091/mbc.E11-02-0139 |
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author | Kim, Woong Matsui, Takaaki Yamao, Masataka Ishibashi, Makoto Tamada, Kota Takumi, Toru Kohno, Kenji Oba, Shigeyuki Ishii, Shin Sakumura, Yuichi Bessho, Yasumasa |
author_facet | Kim, Woong Matsui, Takaaki Yamao, Masataka Ishibashi, Makoto Tamada, Kota Takumi, Toru Kohno, Kenji Oba, Shigeyuki Ishii, Shin Sakumura, Yuichi Bessho, Yasumasa |
author_sort | Kim, Woong |
collection | PubMed |
description | The number of vertebrae is defined strictly for a given species and depends on the number of somites, which are the earliest metameric structures that form in development. Somites are formed by sequential segmentation. The periodicity of somite segmentation is orchestrated by the synchronous oscillation of gene expression in the presomitic mesoderm (PSM), termed the “somite segmentation clock,” in which Notch signaling plays a crucial role. Here we show that the clock period is sensitive to Notch activity, which is fine-tuned by its feedback regulator, Notch-regulated ankyrin repeat protein (Nrarp), and that Nrarp is essential for forming the proper number and morphology of axial skeleton components. Null-mutant mice for Nrarp have fewer vertebrae and have defective morphologies. Notch activity is enhanced in the PSM of the Nrarp(−/–) embryo, where the ∼2-h segmentation period is extended by 5 min, thereby forming fewer somites and their resultant vertebrae. Reduced Notch activity partially rescues the Nrarp(−/–) phenotype in the number of somites, but not in morphology. Therefore we propose that the period of the somite segmentation clock is sensitive to Notch activity and that Nrarp plays essential roles in the morphology of vertebrae and ribs. |
format | Online Article Text |
id | pubmed-3172277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31722772011-11-30 The period of the somite segmentation clock is sensitive to Notch activity Kim, Woong Matsui, Takaaki Yamao, Masataka Ishibashi, Makoto Tamada, Kota Takumi, Toru Kohno, Kenji Oba, Shigeyuki Ishii, Shin Sakumura, Yuichi Bessho, Yasumasa Mol Biol Cell Articles The number of vertebrae is defined strictly for a given species and depends on the number of somites, which are the earliest metameric structures that form in development. Somites are formed by sequential segmentation. The periodicity of somite segmentation is orchestrated by the synchronous oscillation of gene expression in the presomitic mesoderm (PSM), termed the “somite segmentation clock,” in which Notch signaling plays a crucial role. Here we show that the clock period is sensitive to Notch activity, which is fine-tuned by its feedback regulator, Notch-regulated ankyrin repeat protein (Nrarp), and that Nrarp is essential for forming the proper number and morphology of axial skeleton components. Null-mutant mice for Nrarp have fewer vertebrae and have defective morphologies. Notch activity is enhanced in the PSM of the Nrarp(−/–) embryo, where the ∼2-h segmentation period is extended by 5 min, thereby forming fewer somites and their resultant vertebrae. Reduced Notch activity partially rescues the Nrarp(−/–) phenotype in the number of somites, but not in morphology. Therefore we propose that the period of the somite segmentation clock is sensitive to Notch activity and that Nrarp plays essential roles in the morphology of vertebrae and ribs. The American Society for Cell Biology 2011-09-15 /pmc/articles/PMC3172277/ /pubmed/21795391 http://dx.doi.org/10.1091/mbc.E11-02-0139 Text en © 2011 Kim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Kim, Woong Matsui, Takaaki Yamao, Masataka Ishibashi, Makoto Tamada, Kota Takumi, Toru Kohno, Kenji Oba, Shigeyuki Ishii, Shin Sakumura, Yuichi Bessho, Yasumasa The period of the somite segmentation clock is sensitive to Notch activity |
title | The period of the somite segmentation clock is sensitive to Notch activity |
title_full | The period of the somite segmentation clock is sensitive to Notch activity |
title_fullStr | The period of the somite segmentation clock is sensitive to Notch activity |
title_full_unstemmed | The period of the somite segmentation clock is sensitive to Notch activity |
title_short | The period of the somite segmentation clock is sensitive to Notch activity |
title_sort | period of the somite segmentation clock is sensitive to notch activity |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172277/ https://www.ncbi.nlm.nih.gov/pubmed/21795391 http://dx.doi.org/10.1091/mbc.E11-02-0139 |
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