Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses

It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause asept...

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Autores principales: Hasib, Lekbira, Dilcher, Meik, Hufert, Frank, König-Meyer, Ursula, Weidmann, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Humana Press Inc 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172416/
https://www.ncbi.nlm.nih.gov/pubmed/21390485
http://dx.doi.org/10.1007/s12033-011-9389-3
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author Hasib, Lekbira
Dilcher, Meik
Hufert, Frank
König-Meyer, Ursula
Weidmann, Manfred
author_facet Hasib, Lekbira
Dilcher, Meik
Hufert, Frank
König-Meyer, Ursula
Weidmann, Manfred
author_sort Hasib, Lekbira
collection PubMed
description It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses.
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spelling pubmed-31724162011-09-26 Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses Hasib, Lekbira Dilcher, Meik Hufert, Frank König-Meyer, Ursula Weidmann, Manfred Mol Biotechnol Research It is suspected that apart from tick-borne encephalitis virus several additional European Arboviruses such as the sandfly borne Toscana virus, sandfly fever Sicilian virus and sandfly fever Naples virus, mosquito-borne Tahyna virus, Inkoo virus, Batai virus and tick-borne Uukuniemi virus cause aseptic meningo-encephalitis or febrile disease in Europe. Currently, the microarray technology is developing rapidly and there are many efforts to apply it to infectious diseases diagnostics. In order to arrive at an assay system useful for high throughput analysis of samples from aseptic meningo-encephalitis cases the authors developed a combined multiplex ligation-dependent probe amplification and flow-through microarray assay for the detection of European Bunyaviruses. These results show that this combined assay indeed is highly sensitive, and specific for the accurate detection of multiple viruses. Humana Press Inc 2011-03-10 2011 /pmc/articles/PMC3172416/ /pubmed/21390485 http://dx.doi.org/10.1007/s12033-011-9389-3 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Research
Hasib, Lekbira
Dilcher, Meik
Hufert, Frank
König-Meyer, Ursula
Weidmann, Manfred
Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title_full Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title_fullStr Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title_full_unstemmed Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title_short Development of a Flow-Trough Microarray based Reverse Transcriptase Multiplex Ligation-Dependent Probe Amplification Assay for the Detection of European Bunyaviruses
title_sort development of a flow-trough microarray based reverse transcriptase multiplex ligation-dependent probe amplification assay for the detection of european bunyaviruses
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172416/
https://www.ncbi.nlm.nih.gov/pubmed/21390485
http://dx.doi.org/10.1007/s12033-011-9389-3
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