SAGE is far more sensitive than EST for detecting low-abundance transcripts

BACKGROUND: Isolation of low-abundance transcripts expressed in a genome remains a serious challenge in transcriptome studies. The sensitivity of the methods used for analysis has a direct impact on the efficiency of the detection. We compared the EST method and the SAGE method to determine which on...

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Detalles Bibliográficos
Autores principales: Sun, Miao, Zhou, Guolin, Lee, Sanggyu, Chen, Jianjun, Shi, Run Zhang, Wang, San Ming
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317289/
https://www.ncbi.nlm.nih.gov/pubmed/14704093
http://dx.doi.org/10.1186/1471-2164-5-1
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author Sun, Miao
Zhou, Guolin
Lee, Sanggyu
Chen, Jianjun
Shi, Run Zhang
Wang, San Ming
author_facet Sun, Miao
Zhou, Guolin
Lee, Sanggyu
Chen, Jianjun
Shi, Run Zhang
Wang, San Ming
author_sort Sun, Miao
collection PubMed
description BACKGROUND: Isolation of low-abundance transcripts expressed in a genome remains a serious challenge in transcriptome studies. The sensitivity of the methods used for analysis has a direct impact on the efficiency of the detection. We compared the EST method and the SAGE method to determine which one is more sensitive and to what extent the sensitivity is great for the detection of low-abundance transcripts. RESULTS: Using the same low-abundance transcripts detected by both methods as the targeted sequences, we observed that the SAGE method is 26 times more sensitive than the EST method for the detection of low-abundance transcripts. CONCLUSIONS: The SAGE method is more efficient than the EST method in detecting the low-abundance transcripts.
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spelling pubmed-3172892004-01-23 SAGE is far more sensitive than EST for detecting low-abundance transcripts Sun, Miao Zhou, Guolin Lee, Sanggyu Chen, Jianjun Shi, Run Zhang Wang, San Ming BMC Genomics Research Article BACKGROUND: Isolation of low-abundance transcripts expressed in a genome remains a serious challenge in transcriptome studies. The sensitivity of the methods used for analysis has a direct impact on the efficiency of the detection. We compared the EST method and the SAGE method to determine which one is more sensitive and to what extent the sensitivity is great for the detection of low-abundance transcripts. RESULTS: Using the same low-abundance transcripts detected by both methods as the targeted sequences, we observed that the SAGE method is 26 times more sensitive than the EST method for the detection of low-abundance transcripts. CONCLUSIONS: The SAGE method is more efficient than the EST method in detecting the low-abundance transcripts. BioMed Central 2004-01-05 /pmc/articles/PMC317289/ /pubmed/14704093 http://dx.doi.org/10.1186/1471-2164-5-1 Text en Copyright © 2004 Sun et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Sun, Miao
Zhou, Guolin
Lee, Sanggyu
Chen, Jianjun
Shi, Run Zhang
Wang, San Ming
SAGE is far more sensitive than EST for detecting low-abundance transcripts
title SAGE is far more sensitive than EST for detecting low-abundance transcripts
title_full SAGE is far more sensitive than EST for detecting low-abundance transcripts
title_fullStr SAGE is far more sensitive than EST for detecting low-abundance transcripts
title_full_unstemmed SAGE is far more sensitive than EST for detecting low-abundance transcripts
title_short SAGE is far more sensitive than EST for detecting low-abundance transcripts
title_sort sage is far more sensitive than est for detecting low-abundance transcripts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317289/
https://www.ncbi.nlm.nih.gov/pubmed/14704093
http://dx.doi.org/10.1186/1471-2164-5-1
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AT chenjianjun sageisfarmoresensitivethanestfordetectinglowabundancetranscripts
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AT wangsanming sageisfarmoresensitivethanestfordetectinglowabundancetranscripts

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