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Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reacti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172980/ https://www.ncbi.nlm.nih.gov/pubmed/21922037 http://dx.doi.org/10.1155/2011/282739 |
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author | Perrotta, Ida Carito, Valentina Russo, Emilio Tripepi, Sandro Aquila, Saveria Donato, Giuseppe |
author_facet | Perrotta, Ida Carito, Valentina Russo, Emilio Tripepi, Sandro Aquila, Saveria Donato, Giuseppe |
author_sort | Perrotta, Ida |
collection | PubMed |
description | The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells. |
format | Online Article Text |
id | pubmed-3172980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31729802011-09-15 Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study Perrotta, Ida Carito, Valentina Russo, Emilio Tripepi, Sandro Aquila, Saveria Donato, Giuseppe Oxid Med Cell Longev Research Article The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells. Hindawi Publishing Corporation 2011 2011-09-13 /pmc/articles/PMC3172980/ /pubmed/21922037 http://dx.doi.org/10.1155/2011/282739 Text en Copyright © 2011 Ida Perrotta et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Perrotta, Ida Carito, Valentina Russo, Emilio Tripepi, Sandro Aquila, Saveria Donato, Giuseppe Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title | Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title_full | Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title_fullStr | Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title_full_unstemmed | Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title_short | Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study |
title_sort | macrophage autophagy and oxidative stress: an ultrastructural and immunoelectron microscopical study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172980/ https://www.ncbi.nlm.nih.gov/pubmed/21922037 http://dx.doi.org/10.1155/2011/282739 |
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