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Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study

The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reacti...

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Autores principales: Perrotta, Ida, Carito, Valentina, Russo, Emilio, Tripepi, Sandro, Aquila, Saveria, Donato, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172980/
https://www.ncbi.nlm.nih.gov/pubmed/21922037
http://dx.doi.org/10.1155/2011/282739
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author Perrotta, Ida
Carito, Valentina
Russo, Emilio
Tripepi, Sandro
Aquila, Saveria
Donato, Giuseppe
author_facet Perrotta, Ida
Carito, Valentina
Russo, Emilio
Tripepi, Sandro
Aquila, Saveria
Donato, Giuseppe
author_sort Perrotta, Ida
collection PubMed
description The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells.
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spelling pubmed-31729802011-09-15 Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study Perrotta, Ida Carito, Valentina Russo, Emilio Tripepi, Sandro Aquila, Saveria Donato, Giuseppe Oxid Med Cell Longev Research Article The word autophagy broadly refers to the cellular catabolic processes that lead to the removal of damaged cytosolic proteins or cell organelles through lysosomes. Although autophagy is often observed during programmed cell death, it may also serve as a cell survival mechanism. Accumulation of reactive oxygen species within tissues and cells induces various defense mechanisms or programmed cell death. It has been shown that, besides inducing apoptosis, oxidative stress can also induce autophagy. To date, however, the regulation of autophagy in response to oxidative stress remains largely elusive and poorly understood. Therefore, the present study was designed to examine the ratio between oxidative stress and autophagy in macrophages after oxidant exposure (AAPH) and to investigate the ultrastructural localization of beclin-1, a protein essential for autophagy, under basal and stressful conditions. Our data provide evidence that oxidative stress induces autophagy in macrophages. We demonstrate, for the first time by immunoelectron microscopy, the subcellular localization of beclin-1 in autophagic cells. Hindawi Publishing Corporation 2011 2011-09-13 /pmc/articles/PMC3172980/ /pubmed/21922037 http://dx.doi.org/10.1155/2011/282739 Text en Copyright © 2011 Ida Perrotta et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Perrotta, Ida
Carito, Valentina
Russo, Emilio
Tripepi, Sandro
Aquila, Saveria
Donato, Giuseppe
Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title_full Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title_fullStr Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title_full_unstemmed Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title_short Macrophage Autophagy and Oxidative Stress: An Ultrastructural and Immunoelectron Microscopical Study
title_sort macrophage autophagy and oxidative stress: an ultrastructural and immunoelectron microscopical study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172980/
https://www.ncbi.nlm.nih.gov/pubmed/21922037
http://dx.doi.org/10.1155/2011/282739
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