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A new reverse transcription-polymerase chain reaction method for accurate quantification

BACKGROUND: Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. Specific genes ha...

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Detalles Bibliográficos
Autor principal:	Shiao, Yih-Horng
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2003
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317330/ https://www.ncbi.nlm.nih.gov/pubmed/14664723 http://dx.doi.org/10.1186/1472-6750-3-22

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