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Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173354/ https://www.ncbi.nlm.nih.gov/pubmed/21798065 http://dx.doi.org/10.1186/1756-9966-30-71 |
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author | Luo, Jie Sun, Xin Gao, Feng Zhao, Xiaoliang Zhong, Biao Wang, Hong Sun, Zhijun |
author_facet | Luo, Jie Sun, Xin Gao, Feng Zhao, Xiaoliang Zhong, Biao Wang, Hong Sun, Zhijun |
author_sort | Luo, Jie |
collection | PubMed |
description | OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. RESULTS: 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. CONCLUSION: Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin. |
format | Online Article Text |
id | pubmed-3173354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31733542011-09-15 Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK Luo, Jie Sun, Xin Gao, Feng Zhao, Xiaoliang Zhong, Biao Wang, Hong Sun, Zhijun J Exp Clin Cancer Res Research OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. RESULTS: 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. CONCLUSION: Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin. BioMed Central 2011-07-29 /pmc/articles/PMC3173354/ /pubmed/21798065 http://dx.doi.org/10.1186/1756-9966-30-71 Text en Copyright ©2011 Luo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Luo, Jie Sun, Xin Gao, Feng Zhao, Xiaoliang Zhong, Biao Wang, Hong Sun, Zhijun Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title | Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title_full | Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title_fullStr | Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title_full_unstemmed | Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title_short | Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK |
title_sort | effects of ulinastatin and docetaxel on breast cancer invasion and expression of upa, upar and erk |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173354/ https://www.ncbi.nlm.nih.gov/pubmed/21798065 http://dx.doi.org/10.1186/1756-9966-30-71 |
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