Cargando…

Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK

OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Jie, Sun, Xin, Gao, Feng, Zhao, Xiaoliang, Zhong, Biao, Wang, Hong, Sun, Zhijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173354/
https://www.ncbi.nlm.nih.gov/pubmed/21798065
http://dx.doi.org/10.1186/1756-9966-30-71
_version_ 1782211948239126528
author Luo, Jie
Sun, Xin
Gao, Feng
Zhao, Xiaoliang
Zhong, Biao
Wang, Hong
Sun, Zhijun
author_facet Luo, Jie
Sun, Xin
Gao, Feng
Zhao, Xiaoliang
Zhong, Biao
Wang, Hong
Sun, Zhijun
author_sort Luo, Jie
collection PubMed
description OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. RESULTS: 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. CONCLUSION: Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin.
format Online
Article
Text
id pubmed-3173354
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-31733542011-09-15 Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK Luo, Jie Sun, Xin Gao, Feng Zhao, Xiaoliang Zhong, Biao Wang, Hong Sun, Zhijun J Exp Clin Cancer Res Research OBJECTIVE: To investigate the effects of ulinastatin and docetaxel on invasion of breast cancer cells and expression of uPA, uPAR and ERK, breast cancer MDA-MB-231 and MCF-7 cells. METHODS: The nude mice were treated with PBS, ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively. Their effects on 1) cell invasion ability was assayed using Transwell; 2) expression of uPA, uPAR and ERK was detected by real time PCR and Western blot; 3) uPA, uPAR and p-ERK protein level in nude mice was quantified by immunohistochemistry. RESULTS: 1) Treatment with ulinastatin, docetaxel, and ulinastatin plus docetaxel, respectively, significantly inhibited MDA-MB-231 and MCF-7 cell invasion; 2) mRNA and protein levels of uPA, uPAR and ERK1/2 were inhibited by ulinastatin, but enhanced by docetaxel. CONCLUSION: Ulinastatin can enhance the effects of docetaxel on invasion of breast cancer cells. And that uPA, uPAR and p-ERK expression is obviously inhibited by ulinastatin. BioMed Central 2011-07-29 /pmc/articles/PMC3173354/ /pubmed/21798065 http://dx.doi.org/10.1186/1756-9966-30-71 Text en Copyright ©2011 Luo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Luo, Jie
Sun, Xin
Gao, Feng
Zhao, Xiaoliang
Zhong, Biao
Wang, Hong
Sun, Zhijun
Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title_full Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title_fullStr Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title_full_unstemmed Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title_short Effects of ulinastatin and docetaxel on breast cancer invasion and expression of uPA, uPAR and ERK
title_sort effects of ulinastatin and docetaxel on breast cancer invasion and expression of upa, upar and erk
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173354/
https://www.ncbi.nlm.nih.gov/pubmed/21798065
http://dx.doi.org/10.1186/1756-9966-30-71
work_keys_str_mv AT luojie effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT sunxin effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT gaofeng effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT zhaoxiaoliang effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT zhongbiao effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT wanghong effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk
AT sunzhijun effectsofulinastatinanddocetaxelonbreastcancerinvasionandexpressionofupauparanderk