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Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1

Resolution of inflammation is an active temporally orchestrated process demonstrated by the biosynthesis of novel proresolving mediators. Dysregulation of resolution pathways may underlie prevalent human inflammatory diseases such as cardiovascular diseases and periodontitis. Localized Aggressive Pe...

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Autores principales: Fredman, Gabrielle, Oh, Sungwhan F., Ayilavarapu, Srinivas, Hasturk, Hatice, Serhan, Charles N., Van Dyke, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173372/
https://www.ncbi.nlm.nih.gov/pubmed/21935407
http://dx.doi.org/10.1371/journal.pone.0024422
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author Fredman, Gabrielle
Oh, Sungwhan F.
Ayilavarapu, Srinivas
Hasturk, Hatice
Serhan, Charles N.
Van Dyke, Thomas E.
author_facet Fredman, Gabrielle
Oh, Sungwhan F.
Ayilavarapu, Srinivas
Hasturk, Hatice
Serhan, Charles N.
Van Dyke, Thomas E.
author_sort Fredman, Gabrielle
collection PubMed
description Resolution of inflammation is an active temporally orchestrated process demonstrated by the biosynthesis of novel proresolving mediators. Dysregulation of resolution pathways may underlie prevalent human inflammatory diseases such as cardiovascular diseases and periodontitis. Localized Aggressive Periodontitis (LAP) is an early onset, rapidly progressing form of inflammatory periodontal disease. Here, we report increased surface P-selectin on circulating LAP platelets, and elevated integrin (CD18) surface expression on neutrophils and monocytes compared to healthy, asymptomatic controls. Significantly more platelet-neutrophil and platelet-monocyte aggregates were identified in circulating whole blood of LAP patients compared with asymptomatic controls. LAP whole blood generates increased pro-inflammatory LTB4 with addition of divalent cation ionophore A23187 (5 µM) and significantly less, 15-HETE, 12-HETE, 14-HDHA, and lipoxin A(4). Macrophages from LAP subjects exhibit reduced phagocytosis. The pro-resolving lipid mediator, Resolvin E1 (0.1–100 nM), rescues the impaired phagocytic activity in LAP macrophages. These abnormalities suggest compromised resolution pathways, which may contribute to persistent inflammation resulting in establishment of a chronic inflammatory lesion and periodontal disease progression.
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spelling pubmed-31733722011-09-20 Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1 Fredman, Gabrielle Oh, Sungwhan F. Ayilavarapu, Srinivas Hasturk, Hatice Serhan, Charles N. Van Dyke, Thomas E. PLoS One Research Article Resolution of inflammation is an active temporally orchestrated process demonstrated by the biosynthesis of novel proresolving mediators. Dysregulation of resolution pathways may underlie prevalent human inflammatory diseases such as cardiovascular diseases and periodontitis. Localized Aggressive Periodontitis (LAP) is an early onset, rapidly progressing form of inflammatory periodontal disease. Here, we report increased surface P-selectin on circulating LAP platelets, and elevated integrin (CD18) surface expression on neutrophils and monocytes compared to healthy, asymptomatic controls. Significantly more platelet-neutrophil and platelet-monocyte aggregates were identified in circulating whole blood of LAP patients compared with asymptomatic controls. LAP whole blood generates increased pro-inflammatory LTB4 with addition of divalent cation ionophore A23187 (5 µM) and significantly less, 15-HETE, 12-HETE, 14-HDHA, and lipoxin A(4). Macrophages from LAP subjects exhibit reduced phagocytosis. The pro-resolving lipid mediator, Resolvin E1 (0.1–100 nM), rescues the impaired phagocytic activity in LAP macrophages. These abnormalities suggest compromised resolution pathways, which may contribute to persistent inflammation resulting in establishment of a chronic inflammatory lesion and periodontal disease progression. Public Library of Science 2011-09-14 /pmc/articles/PMC3173372/ /pubmed/21935407 http://dx.doi.org/10.1371/journal.pone.0024422 Text en Fredman et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fredman, Gabrielle
Oh, Sungwhan F.
Ayilavarapu, Srinivas
Hasturk, Hatice
Serhan, Charles N.
Van Dyke, Thomas E.
Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title_full Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title_fullStr Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title_full_unstemmed Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title_short Impaired Phagocytosis in Localized Aggressive Periodontitis: Rescue by Resolvin E1
title_sort impaired phagocytosis in localized aggressive periodontitis: rescue by resolvin e1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173372/
https://www.ncbi.nlm.nih.gov/pubmed/21935407
http://dx.doi.org/10.1371/journal.pone.0024422
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