Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa

The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at lo...

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Autores principales: Ochong, Edwin, Nzila, Alexis, Kimani, Serah, Kokwaro, Gilbert, Mutabingwa, Theonest, Watkins, William, Marsh, Kevin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317348/
https://www.ncbi.nlm.nih.gov/pubmed/14675491
http://dx.doi.org/10.1186/1475-2875-2-46
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author Ochong, Edwin
Nzila, Alexis
Kimani, Serah
Kokwaro, Gilbert
Mutabingwa, Theonest
Watkins, William
Marsh, Kevin
author_facet Ochong, Edwin
Nzila, Alexis
Kimani, Serah
Kokwaro, Gilbert
Mutabingwa, Theonest
Watkins, William
Marsh, Kevin
author_sort Ochong, Edwin
collection PubMed
description The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998–1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation.
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spelling pubmed-3173482004-01-23 Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa Ochong, Edwin Nzila, Alexis Kimani, Serah Kokwaro, Gilbert Mutabingwa, Theonest Watkins, William Marsh, Kevin Malar J Research The selection of point mutation at codon 164 (from isoleucine to leucine) of the dihydrofolate reductase (DHFR) enzyme in Plasmodium falciparum is associated with high sulfadoxine /pyrimethamine (SP) resistance. Using the yeast expression system that allows the detection of dhfr allele present at low level, the presence of this mutation had previously been reported between 1998–1999 in Muheza, Tanzania, an area of high SP resistance. Eighty five P. falciparum isolates, obtained from the same area between 2002 and 2003, were analysed for the presence of Leu-164 mutation, using standard protocol based on PCR-RFLP. None of the isolates had the Leu-164 mutation. BioMed Central 2003-12-15 /pmc/articles/PMC317348/ /pubmed/14675491 http://dx.doi.org/10.1186/1475-2875-2-46 Text en Copyright © 2003 Ochong et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research
Ochong, Edwin
Nzila, Alexis
Kimani, Serah
Kokwaro, Gilbert
Mutabingwa, Theonest
Watkins, William
Marsh, Kevin
Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title_full Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title_fullStr Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title_full_unstemmed Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title_short Molecular monitoring of the Leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in Africa
title_sort molecular monitoring of the leu-164 mutation of dihydrofolate reductase in a highly sulfadoxine/pyrimethamine-resistant area in africa
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC317348/
https://www.ncbi.nlm.nih.gov/pubmed/14675491
http://dx.doi.org/10.1186/1475-2875-2-46
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