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The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model

The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a...

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Autores principales: Zaura, Egija, Buijs, Mark J., Hoogenkamp, Michel A., Ciric, Lena, Papetti, Adele, Signoretto, Caterina, Stauder, Monica, Lingström, Peter, Pratten, Jonathan, Spratt, David A., Wilson, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173737/
https://www.ncbi.nlm.nih.gov/pubmed/21941428
http://dx.doi.org/10.1155/2011/135034
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author Zaura, Egija
Buijs, Mark J.
Hoogenkamp, Michel A.
Ciric, Lena
Papetti, Adele
Signoretto, Caterina
Stauder, Monica
Lingström, Peter
Pratten, Jonathan
Spratt, David A.
Wilson, Michael
author_facet Zaura, Egija
Buijs, Mark J.
Hoogenkamp, Michel A.
Ciric, Lena
Papetti, Adele
Signoretto, Caterina
Stauder, Monica
Lingström, Peter
Pratten, Jonathan
Spratt, David A.
Wilson, Michael
author_sort Zaura, Egija
collection PubMed
description The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential.
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spelling pubmed-31737372011-09-22 The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model Zaura, Egija Buijs, Mark J. Hoogenkamp, Michel A. Ciric, Lena Papetti, Adele Signoretto, Caterina Stauder, Monica Lingström, Peter Pratten, Jonathan Spratt, David A. Wilson, Michael J Biomed Biotechnol Research Article The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential. Hindawi Publishing Corporation 2011 2011-09-14 /pmc/articles/PMC3173737/ /pubmed/21941428 http://dx.doi.org/10.1155/2011/135034 Text en Copyright © 2011 Egija Zaura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zaura, Egija
Buijs, Mark J.
Hoogenkamp, Michel A.
Ciric, Lena
Papetti, Adele
Signoretto, Caterina
Stauder, Monica
Lingström, Peter
Pratten, Jonathan
Spratt, David A.
Wilson, Michael
The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title_full The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title_fullStr The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title_full_unstemmed The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title_short The Effects of Fractions from Shiitake Mushroom on Composition and Cariogenicity of Dental Plaque Microcosms in an In Vitro Caries Model
title_sort effects of fractions from shiitake mushroom on composition and cariogenicity of dental plaque microcosms in an in vitro caries model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173737/
https://www.ncbi.nlm.nih.gov/pubmed/21941428
http://dx.doi.org/10.1155/2011/135034
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