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Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers
We compared structure and function of EDL and Soleus muscles in adult (4–6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca(2+)-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173739/ https://www.ncbi.nlm.nih.gov/pubmed/21941434 http://dx.doi.org/10.1155/2011/634075 |
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author | Paolini, Cecilia Quarta, Marco D'Onofrio, Laura Reggiani, Carlo Protasi, Feliciano |
author_facet | Paolini, Cecilia Quarta, Marco D'Onofrio, Laura Reggiani, Carlo Protasi, Feliciano |
author_sort | Paolini, Cecilia |
collection | PubMed |
description | We compared structure and function of EDL and Soleus muscles in adult (4–6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca(2+)-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70–80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca(2+) released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca(2+) amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca(2+) leak is more pronounced. |
format | Online Article Text |
id | pubmed-3173739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31737392011-09-22 Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers Paolini, Cecilia Quarta, Marco D'Onofrio, Laura Reggiani, Carlo Protasi, Feliciano J Biomed Biotechnol Research Article We compared structure and function of EDL and Soleus muscles in adult (4–6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca(2+)-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70–80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca(2+) released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca(2+) amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca(2+) leak is more pronounced. Hindawi Publishing Corporation 2011 2011-09-14 /pmc/articles/PMC3173739/ /pubmed/21941434 http://dx.doi.org/10.1155/2011/634075 Text en Copyright © 2011 Cecilia Paolini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Paolini, Cecilia Quarta, Marco D'Onofrio, Laura Reggiani, Carlo Protasi, Feliciano Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title | Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title_full | Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title_fullStr | Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title_full_unstemmed | Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title_short | Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers |
title_sort | differential effect of calsequestrin ablation on structure and function of fast and slow skeletal muscle fibers |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173739/ https://www.ncbi.nlm.nih.gov/pubmed/21941434 http://dx.doi.org/10.1155/2011/634075 |
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