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Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells

BACKGROUND: Phyllanthus (Euphorbiaceae) species have long been used in folk medicine to treat various pathological conditions including liver diseases. Some species of Phyllanthus were found to exhibit hepatoprotective activity against drugs or toxins and this property was majorly attributed to phyl...

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Detalles Bibliográficos
Autores principales: Sharma, Surendra Kumar, Arogya, Sheela Meruga, Bhaskarmurthy, Deepak Hiraganahalli, Agarwal, Amit, Velusami, Chandrasekaran Chinampudar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications Pvt Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173898/
https://www.ncbi.nlm.nih.gov/pubmed/21969794
http://dx.doi.org/10.4103/0973-1296.84237
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author Sharma, Surendra Kumar
Arogya, Sheela Meruga
Bhaskarmurthy, Deepak Hiraganahalli
Agarwal, Amit
Velusami, Chandrasekaran Chinampudar
author_facet Sharma, Surendra Kumar
Arogya, Sheela Meruga
Bhaskarmurthy, Deepak Hiraganahalli
Agarwal, Amit
Velusami, Chandrasekaran Chinampudar
author_sort Sharma, Surendra Kumar
collection PubMed
description BACKGROUND: Phyllanthus (Euphorbiaceae) species have long been used in folk medicine to treat various pathological conditions including liver diseases. Some species of Phyllanthus were found to exhibit hepatoprotective activity against drugs or toxins and this property was majorly attributed to phyllanthin and hypophyllanthin. In this study, we examined the hepatoprotective activity of five different species of Phyllanthus, namely, Phyllanthus amarus, Phyllanthus fraternus, Phyllanthus maderaspatensis, Phyllanthus urinaria, and Phyllanthus Rotundifolius. The extracts were also evaluated for the presence of key phytoconstituents, phyllanthin and hypophyllanthin. MATERIALS AND METHODS: The extracts were evaluated for hepatoprotective activity against tert-butyl hydroxide (t-BH)-induced cytotoxicity using human hepatocarcinoma cells (HepG2 cell line). RESULTS: Only P. urinaria and P. maderaspatensis exhibited significant hepatoprotective activity as evident from increased cell viability. The HPLC profile revealed that except P. amarus, the other extracts did not contain phyllanthin and hypophyllanthin. CONCLUSION: P. urinaria and P. maderaspatensis demonstrated dose-dependent hepatoprotective activity and hence, can provide promising therapeutic interventions against chemical–induced liver damage.
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spelling pubmed-31738982011-10-03 Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells Sharma, Surendra Kumar Arogya, Sheela Meruga Bhaskarmurthy, Deepak Hiraganahalli Agarwal, Amit Velusami, Chandrasekaran Chinampudar Pharmacogn Mag Original Article BACKGROUND: Phyllanthus (Euphorbiaceae) species have long been used in folk medicine to treat various pathological conditions including liver diseases. Some species of Phyllanthus were found to exhibit hepatoprotective activity against drugs or toxins and this property was majorly attributed to phyllanthin and hypophyllanthin. In this study, we examined the hepatoprotective activity of five different species of Phyllanthus, namely, Phyllanthus amarus, Phyllanthus fraternus, Phyllanthus maderaspatensis, Phyllanthus urinaria, and Phyllanthus Rotundifolius. The extracts were also evaluated for the presence of key phytoconstituents, phyllanthin and hypophyllanthin. MATERIALS AND METHODS: The extracts were evaluated for hepatoprotective activity against tert-butyl hydroxide (t-BH)-induced cytotoxicity using human hepatocarcinoma cells (HepG2 cell line). RESULTS: Only P. urinaria and P. maderaspatensis exhibited significant hepatoprotective activity as evident from increased cell viability. The HPLC profile revealed that except P. amarus, the other extracts did not contain phyllanthin and hypophyllanthin. CONCLUSION: P. urinaria and P. maderaspatensis demonstrated dose-dependent hepatoprotective activity and hence, can provide promising therapeutic interventions against chemical–induced liver damage. Medknow Publications Pvt Ltd 2011 /pmc/articles/PMC3173898/ /pubmed/21969794 http://dx.doi.org/10.4103/0973-1296.84237 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Sharma, Surendra Kumar
Arogya, Sheela Meruga
Bhaskarmurthy, Deepak Hiraganahalli
Agarwal, Amit
Velusami, Chandrasekaran Chinampudar
Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title_full Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title_fullStr Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title_full_unstemmed Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title_short Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells
title_sort hepatoprotective activity of the phyllanthus species on tert-butyl hydroperoxide (t-bh)-induced cytotoxicity in hepg2 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173898/
https://www.ncbi.nlm.nih.gov/pubmed/21969794
http://dx.doi.org/10.4103/0973-1296.84237
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