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Circadian aspects of hyperthermia in mice induced by Aconitum napellus

BACKGROUND: Aconitum napellus (Acn) is used topically to relieve pain, itching and inflammation, and internally to reduce febrile states, among others. Any circadian time-related consequences of Acn administration are unknown. The objective of this study was to explore the effects of two doses of Ac...

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Autores principales: de la Peña, Salvador Sánchez, Sothern, Robert B., López, Fernando Santillán, Lujambio, Irene Mendoza, Waizel-Bucay, José, Sánchez, Carolina Olarte, Monroy, Claudia Pérez, Betancourt, Eduardo Tena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications Pvt Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173899/
https://www.ncbi.nlm.nih.gov/pubmed/21969795
http://dx.doi.org/10.4103/0973-1296.84238
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author de la Peña, Salvador Sánchez
Sothern, Robert B.
López, Fernando Santillán
Lujambio, Irene Mendoza
Waizel-Bucay, José
Sánchez, Carolina Olarte
Monroy, Claudia Pérez
Betancourt, Eduardo Tena
author_facet de la Peña, Salvador Sánchez
Sothern, Robert B.
López, Fernando Santillán
Lujambio, Irene Mendoza
Waizel-Bucay, José
Sánchez, Carolina Olarte
Monroy, Claudia Pérez
Betancourt, Eduardo Tena
author_sort de la Peña, Salvador Sánchez
collection PubMed
description BACKGROUND: Aconitum napellus (Acn) is used topically to relieve pain, itching and inflammation, and internally to reduce febrile states, among others. Any circadian time-related consequences of Acn administration are unknown. The objective of this study was to explore the effects of two doses of Acn on body temperature (BT) of mice treated at six different times over 24 hours. MATERIALS AND METHODS: BALB/c female mice were housed in six chambers (six mice each) with air temperature 24 ± 3°C, humidity 60 ± 4%, and a 12-hours light (L)/12-hours dark cycle, but with L-onset staggered by 4 hours between chambers so that study at one external test time resulted in six test times (02, 06, 10, 14, 18 and 22 hours [h] after light onset). Rectal temperature (RT; in °C) was measured at baseline (B) and 1 hour after oral treatment with placebo (P) or two doses of Acn (6C and 30C, two studies each) in six studies over an 8 day span. The difference in RT for each mouse from the respective B + P timepoint mean RT was computed following each Acn treatment, and data from each of the six studies (original RT and difference from B + P) were analyzed for time-effect by analysis of variance (ANOVA) and for circadian rhythm by 24-hour cosine fitting. RESULTS: A circadian rhythm in RT was found at B and after P (mean: 35.58°C vs. 35.69°C; peak: 15:31 h vs. 15:40 h) and after each Acn dose (30C or 6C). Acn induced hyperthermia and the overall change in BT was rhythmically significant for each dose (mean = +1.95°C vs. +1.70°C), with greatest hyperthermia observed during the L-span for each dose (peak = 08:56 h vs. 05:17 h). CONCLUSION: Acn administered around the clock induced hyperthermia overall and in a time-dependent manner, with greatest effects during the resting (L) span. Thus, time of day may significantly impact the outcome of Acn and other homeopathic treatments and should be considered in determining optimal dosing and treatment time(s) in order to increase the desired outcome and decrease undesired effects.
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spelling pubmed-31738992011-10-03 Circadian aspects of hyperthermia in mice induced by Aconitum napellus de la Peña, Salvador Sánchez Sothern, Robert B. López, Fernando Santillán Lujambio, Irene Mendoza Waizel-Bucay, José Sánchez, Carolina Olarte Monroy, Claudia Pérez Betancourt, Eduardo Tena Pharmacogn Mag Original Article BACKGROUND: Aconitum napellus (Acn) is used topically to relieve pain, itching and inflammation, and internally to reduce febrile states, among others. Any circadian time-related consequences of Acn administration are unknown. The objective of this study was to explore the effects of two doses of Acn on body temperature (BT) of mice treated at six different times over 24 hours. MATERIALS AND METHODS: BALB/c female mice were housed in six chambers (six mice each) with air temperature 24 ± 3°C, humidity 60 ± 4%, and a 12-hours light (L)/12-hours dark cycle, but with L-onset staggered by 4 hours between chambers so that study at one external test time resulted in six test times (02, 06, 10, 14, 18 and 22 hours [h] after light onset). Rectal temperature (RT; in °C) was measured at baseline (B) and 1 hour after oral treatment with placebo (P) or two doses of Acn (6C and 30C, two studies each) in six studies over an 8 day span. The difference in RT for each mouse from the respective B + P timepoint mean RT was computed following each Acn treatment, and data from each of the six studies (original RT and difference from B + P) were analyzed for time-effect by analysis of variance (ANOVA) and for circadian rhythm by 24-hour cosine fitting. RESULTS: A circadian rhythm in RT was found at B and after P (mean: 35.58°C vs. 35.69°C; peak: 15:31 h vs. 15:40 h) and after each Acn dose (30C or 6C). Acn induced hyperthermia and the overall change in BT was rhythmically significant for each dose (mean = +1.95°C vs. +1.70°C), with greatest hyperthermia observed during the L-span for each dose (peak = 08:56 h vs. 05:17 h). CONCLUSION: Acn administered around the clock induced hyperthermia overall and in a time-dependent manner, with greatest effects during the resting (L) span. Thus, time of day may significantly impact the outcome of Acn and other homeopathic treatments and should be considered in determining optimal dosing and treatment time(s) in order to increase the desired outcome and decrease undesired effects. Medknow Publications Pvt Ltd 2011 /pmc/articles/PMC3173899/ /pubmed/21969795 http://dx.doi.org/10.4103/0973-1296.84238 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
de la Peña, Salvador Sánchez
Sothern, Robert B.
López, Fernando Santillán
Lujambio, Irene Mendoza
Waizel-Bucay, José
Sánchez, Carolina Olarte
Monroy, Claudia Pérez
Betancourt, Eduardo Tena
Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title_full Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title_fullStr Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title_full_unstemmed Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title_short Circadian aspects of hyperthermia in mice induced by Aconitum napellus
title_sort circadian aspects of hyperthermia in mice induced by aconitum napellus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3173899/
https://www.ncbi.nlm.nih.gov/pubmed/21969795
http://dx.doi.org/10.4103/0973-1296.84238
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