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Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines

Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resis...

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Autores principales: Krens, Frans A., Schaart, Jan G., Groenwold, Remmelt, Walraven, A. Evert J., Hesselink, Thamara, Thissen, Jac T. N. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174370/
https://www.ncbi.nlm.nih.gov/pubmed/21243525
http://dx.doi.org/10.1007/s11248-011-9484-z
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author Krens, Frans A.
Schaart, Jan G.
Groenwold, Remmelt
Walraven, A. Evert J.
Hesselink, Thamara
Thissen, Jac T. N. M.
author_facet Krens, Frans A.
Schaart, Jan G.
Groenwold, Remmelt
Walraven, A. Evert J.
Hesselink, Thamara
Thissen, Jac T. N. M.
author_sort Krens, Frans A.
collection PubMed
description Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11248-011-9484-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-31743702011-09-26 Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines Krens, Frans A. Schaart, Jan G. Groenwold, Remmelt Walraven, A. Evert J. Hesselink, Thamara Thissen, Jac T. N. M. Transgenic Res Original Paper Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows the rapid introduction of resistance genes directly into high quality apple cultivars. Resistance genes can be derived from apple itself but genetic modification also opens up the possibility to use other, non-host resistance genes. A prerequisite for application is the long-term performance and stability of the gene annex trait in the field. For this study, we produced and selected a series of transgenic apple lines of two cultivars, i.e. ‘Elstar’ and ‘Gala’ in which the barley hordothionin gene (hth) was introduced. After multiplication, the GM hth-lines, non-GM susceptible and resistant controls and GM non-hth controls were planted in a random block design in a field trial in 40 replicates. Scab resistance was monitored after artificial inoculation (first year) and after natural infection (subsequent years). After the trial period, the level of expression of the hth gene was checked by quantitative RT-PCR. Four of the six GM hth apple lines proved to be significantly less susceptible to apple scab and this trait was found to be stable for the entire 4-year period. Hth expression at the mRNA level was also stable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11248-011-9484-z) contains supplementary material, which is available to authorized users. Springer Netherlands 2011-01-19 2011 /pmc/articles/PMC3174370/ /pubmed/21243525 http://dx.doi.org/10.1007/s11248-011-9484-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Krens, Frans A.
Schaart, Jan G.
Groenwold, Remmelt
Walraven, A. Evert J.
Hesselink, Thamara
Thissen, Jac T. N. M.
Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title_full Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title_fullStr Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title_full_unstemmed Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title_short Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
title_sort performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174370/
https://www.ncbi.nlm.nih.gov/pubmed/21243525
http://dx.doi.org/10.1007/s11248-011-9484-z
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