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Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum

BACKGROUND: Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identification of microorganisms capable of efficient and simultaneous utilization of b...

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Autores principales: Hu, Cuimin, Wu, Siguo, Wang, Qian, Jin, Guojie, Shen, Hongwei, Zhao, Zongbao K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174874/
https://www.ncbi.nlm.nih.gov/pubmed/21864398
http://dx.doi.org/10.1186/1754-6834-4-25
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author Hu, Cuimin
Wu, Siguo
Wang, Qian
Jin, Guojie
Shen, Hongwei
Zhao, Zongbao K
author_facet Hu, Cuimin
Wu, Siguo
Wang, Qian
Jin, Guojie
Shen, Hongwei
Zhao, Zongbao K
author_sort Hu, Cuimin
collection PubMed
description BACKGROUND: Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identification of microorganisms capable of efficient and simultaneous utilization of both glucose and xylose is pivotal to improving this process. RESULTS: In this study, we found that the oleaginous yeast strain Trichosporon cutaneum AS 2.571 assimilated glucose and xylose simultaneously, and accumulated intracellular lipid up to 59 wt% with a lipid coefficient up to 0.17 g/g sugar, upon cultivation on a 2:1 glucose/xylose mixture in a 3-liter stirred-tank bioreactor. In addition, no classic pattern of diauxic growth behavior was seen; the microbial cell mass increased during the whole culture process without any lag periods. In shake-flask cultures with different initial glucose:xylose ratios, glucose and xylose were consumed simultaneously at rates roughly proportional to their individual concentrations in the medium, leading to complete utilization of both sugars at the same time. Simultaneous utilization of glucose and xylose was also seen during fermentation of corn-stover hydrolysate with a lipid content and coefficient of 39.2% and 0.15 g/g sugar, respectively. The lipid produced had a fatty-acid compositional profile similar to those of conventional vegetable oil, indicating that it could have potential as a raw material for biodiesel production. CONCLUSION: Efficient lipid production with simultaneous consumption of glucose and xylose was achieved in this study. This process provides an exciting opportunity to transform lignocellulosic materials into biofuel molecules, and should also encourage further study to elucidate this unique sugar-assimilation mechanism.
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spelling pubmed-31748742011-09-17 Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum Hu, Cuimin Wu, Siguo Wang, Qian Jin, Guojie Shen, Hongwei Zhao, Zongbao K Biotechnol Biofuels Research BACKGROUND: Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identification of microorganisms capable of efficient and simultaneous utilization of both glucose and xylose is pivotal to improving this process. RESULTS: In this study, we found that the oleaginous yeast strain Trichosporon cutaneum AS 2.571 assimilated glucose and xylose simultaneously, and accumulated intracellular lipid up to 59 wt% with a lipid coefficient up to 0.17 g/g sugar, upon cultivation on a 2:1 glucose/xylose mixture in a 3-liter stirred-tank bioreactor. In addition, no classic pattern of diauxic growth behavior was seen; the microbial cell mass increased during the whole culture process without any lag periods. In shake-flask cultures with different initial glucose:xylose ratios, glucose and xylose were consumed simultaneously at rates roughly proportional to their individual concentrations in the medium, leading to complete utilization of both sugars at the same time. Simultaneous utilization of glucose and xylose was also seen during fermentation of corn-stover hydrolysate with a lipid content and coefficient of 39.2% and 0.15 g/g sugar, respectively. The lipid produced had a fatty-acid compositional profile similar to those of conventional vegetable oil, indicating that it could have potential as a raw material for biodiesel production. CONCLUSION: Efficient lipid production with simultaneous consumption of glucose and xylose was achieved in this study. This process provides an exciting opportunity to transform lignocellulosic materials into biofuel molecules, and should also encourage further study to elucidate this unique sugar-assimilation mechanism. BioMed Central 2011-08-24 /pmc/articles/PMC3174874/ /pubmed/21864398 http://dx.doi.org/10.1186/1754-6834-4-25 Text en Copyright ©2011 Hu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Hu, Cuimin
Wu, Siguo
Wang, Qian
Jin, Guojie
Shen, Hongwei
Zhao, Zongbao K
Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title_full Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title_fullStr Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title_full_unstemmed Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title_short Simultaneous utilization of glucose and xylose for lipid production by Trichosporon cutaneum
title_sort simultaneous utilization of glucose and xylose for lipid production by trichosporon cutaneum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3174874/
https://www.ncbi.nlm.nih.gov/pubmed/21864398
http://dx.doi.org/10.1186/1754-6834-4-25
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