A combined functional and structural genomics approach identified an EST-SSR marker with complete linkage to the Ligon lintless-2 genetic locus in cotton (Gossypium hirsutum L.)

BACKGROUND: Cotton fiber length is an important quality attribute to the textile industry and longer fibers can be more efficiently spun into yarns to produce superior fabrics. There is typically a negative correlation between yield and fiber quality traits such as length. An understanding of the regulatory mechanisms controlling fiber length can potentially provide a valuable tool for cotton breeders to improve fiber length while maintaining high yields. The cotton (Gossypium hirsutum L.) fiber mutation Ligon lintless-2 is controlled by a single dominant gene (Li(2)) that results in significantly shorter fibers than a wild-type. In a near-isogenic state with a wild-type cotton line, Li(2 )is a model system with which to study fiber elongation. RESULTS: Two near-isogenic lines of Ligon lintless-2 (Li(2)) cotton, one mutant and one wild-type, were developed through five generations of backcrosses (BC(5)). An F(2 )population was developed from a cross between the two Li(2 )near-isogenic lines and used to develop a linkage map of the Li(2 )locus on chromosome 18. Five simple sequence repeat (SSR) markers were closely mapped around the Li(2 )locus region with two of the markers flanking the Li(2 )locus at 0.87 and 0.52 centimorgan. No apparent differences in fiber initiation and early fiber elongation were observed between the mutant ovules and the wild-type ones. Gene expression profiling using microarrays suggested roles of reactive oxygen species (ROS) homeostasis and cytokinin regulation in the Li(2 )mutant phenotype. Microarray gene expression data led to successful identification of an EST-SSR marker (NAU3991) that displayed complete linkage to the Li(2 )locus. CONCLUSIONS: In the field of cotton genomics, we report the first successful conversion of gene expression data into an SSR marker that is associated with a genomic region harboring a gene responsible for a fiber trait. The EST-derived SSR marker NAU3991 displayed complete linkage to the Li(2 )locus on chromosome 18 and resided in a gene with similarity to a putative plectin-related protein. The complete linkage suggests that this expressed sequence may be the Li(2 )gene.