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Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1

BACKGROUND: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy. MET...

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Autores principales: Li, Lei, O, Wai-Sum, Tang, Fai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175455/
https://www.ncbi.nlm.nih.gov/pubmed/21824440
http://dx.doi.org/10.1186/1477-7827-9-111
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author Li, Lei
O, Wai-Sum
Tang, Fai
author_facet Li, Lei
O, Wai-Sum
Tang, Fai
author_sort Li, Lei
collection PubMed
description BACKGROUND: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy. METHODS: The peptide and gene expression level of adrenomedullin in small antral follicles, large antral follicles and CL was studied by real-time RT-PCR and EIA. The effect of ADM treatment on oestradiol production in 5-day follicular culture and on progesterone production from CL of different pregnant stages was measured by EIA. The interaction of ADM and ET-1 in follicles and CL at their gene expression level was studied by real-time RT-PCR. RESULTS: In the rat ovary, the gene expression of Adm increased during development from small antral follicles to large antral follicles and CL. In vitro treatment of preantral follicular culture for 5 days with ADM increased oestradiol production but did not affect follicular growth or ovulation rate. The regulation of progesterone production by ADM in CL in culture was pregnancy-stage dependent, inhibitory at early and late pregnancy but stimulatory at mid-pregnancy, which might contribute to the high progesterone production rate of the CL at mid-pregnancy. Moreover, the interaction between ADM and ET-1 at both the production and functional levels indicates that these two vasoactive peptides may form an important local, fine-tuning regulatory system together with LH and prolactin for progesterone production in rat CL. CONCLUSIONS: As the CL is the major source of progesterone production even after the formation of placenta in rats, ADM may be an important regulator in progesterone production to meet the requirement of pregnancy.
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spelling pubmed-31754552011-09-19 Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1 Li, Lei O, Wai-Sum Tang, Fai Reprod Biol Endocrinol Research BACKGROUND: Adrenomedullin (ADM), a novel vasorelaxant peptide, was found in human/rat ovaries. The present study investigated the interaction of ADM and endothelin-1 (ET-1) in follicles and newly formed corpora lutea (CL) and the actions of ADM on progesterone production in CL during pregnancy. METHODS: The peptide and gene expression level of adrenomedullin in small antral follicles, large antral follicles and CL was studied by real-time RT-PCR and EIA. The effect of ADM treatment on oestradiol production in 5-day follicular culture and on progesterone production from CL of different pregnant stages was measured by EIA. The interaction of ADM and ET-1 in follicles and CL at their gene expression level was studied by real-time RT-PCR. RESULTS: In the rat ovary, the gene expression of Adm increased during development from small antral follicles to large antral follicles and CL. In vitro treatment of preantral follicular culture for 5 days with ADM increased oestradiol production but did not affect follicular growth or ovulation rate. The regulation of progesterone production by ADM in CL in culture was pregnancy-stage dependent, inhibitory at early and late pregnancy but stimulatory at mid-pregnancy, which might contribute to the high progesterone production rate of the CL at mid-pregnancy. Moreover, the interaction between ADM and ET-1 at both the production and functional levels indicates that these two vasoactive peptides may form an important local, fine-tuning regulatory system together with LH and prolactin for progesterone production in rat CL. CONCLUSIONS: As the CL is the major source of progesterone production even after the formation of placenta in rats, ADM may be an important regulator in progesterone production to meet the requirement of pregnancy. BioMed Central 2011-08-09 /pmc/articles/PMC3175455/ /pubmed/21824440 http://dx.doi.org/10.1186/1477-7827-9-111 Text en Copyright ©2011 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Li, Lei
O, Wai-Sum
Tang, Fai
Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title_full Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title_fullStr Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title_full_unstemmed Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title_short Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
title_sort adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175455/
https://www.ncbi.nlm.nih.gov/pubmed/21824440
http://dx.doi.org/10.1186/1477-7827-9-111
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